Abstract
NADH oxidase activity of plasma membranes from rat hepatoma and HeLa cells responded to thiol reagents in a manner different from that of plasma membranes of liver. Specifically, the NADH oxidase activity of plasma membranes of HeLa cells was inhibited by submicromolar concentrations of the thiol reagentsp-chloromercuribenzoate (PCMB),N-ethylmaleimide (NEM), or 5,5′-dithiobis-(2-nitrophenylbenzoic acid) (DTNB), whereas that of the rat liver plasma membranes was unaffected or stimulated over a wide range of concentrations extending into the millimolar range. With some hepatoma preparations, the NADH oxidase activity of hepatoma plasma membranes was stimulated rather than inhibited by PCMB, whereas with all preparations of hepatoma plasma membranes, NEM and DTNB stimulated the activity. In contrast, NADH oxidase activity of rat liver plasma membrane was largely unaffected over the same range of PCMB concentrations that either stimulated or inhibited with rat hepatoma or HeLa cell plasma membranes. Dithiothreitol and glutathione stimulated NADH oxidase activity of plasma membranes of rat liver and hepatoma but inhibited that of HeLa plasma membranes. The findings demonstrate a difference between the NADH oxidase activity of normal rat liver plasma membranes of rat hepatoma and HeLa cell plasma membranes in addition to the differential response to growth factors and hormones reported previously (Brunoet al., 1992). Results are consistent with a structural modification of a NADH oxidase activity involving thiol groups present in plasma membranes of rat hepatoma and HeLa cells but absent or inaccessible with plasma membranes of rat liver.
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References
Aronstam, R. S., Abood, L. G., and Hoss, W. (1978).Mol. Pharmacol. 14, 575–586.
Bennett, C. F., Balcarek, J. M., Varrichio, A., and Crooke, S. T. (1988).Nature 334, 268–270.
Bottari, S., Vauguelin, G., Duriev, O., Klutchko, C., and Strosberg, A. D. (1979).Biochem. Biophys. Res. Commun. 86, 1311–1318.
Brightman, A. O., Wang, J., Miu, R.-K., Sun, I. L., Barr, R., Crane, F. L., and Morré, D. J. (1992).Biochim. Biophys. Acta 1105, 109–117.
Bruno, M., Brightman, A. O., Lawrence, J., Werderitsh, D., Morré, D. M., and Morré, D. J. (1992).Biochem. J. 284, 625–628.
Crane, F. L., Sun, I. L., Clark, M. G., Grebing, C., and Löw, H. (1985).Biochim. Biophys. Acta 811, 233–264.
El Battari, A., Martin, J. M., Luis, J., Pouzol, W., Secchi, J., Marvaldi, J., and Pichon, J. (1988).J. Biol. Chem. 263, 17685–17689.
Ellman, G. L. (1958).Arch. Biochem. Biophys. 74, 433–450.
Falcone, R. C., and Aharony, D. (1990).J. Pharm. Exp. Ther. 255, 565–571.
Holmgren, A. (1985).Annu. Rev. Biochem. 54, 237.
Kloppel, T. M., and Morré, D. J. (1980).J. Natl. Cancer Inst. 64, 1401–1411.
Larsen, N. E., Mullikin-Kilpatrick, D., and Blume, A. J. (1981).Mol. Pharmacol. 20, 255–262.
Maturo, J. M., Hollenberg, M. D., and Aglio, L. S. (1983).Biochemistry 22, 2579–2586.
Morré, D. J. (1971).Methods Enzymol. 22, 130–148.
Morré, D. J., and Brightman, A. O. (1991).J. Bioenerg. Biomembr. 23, 469–489.
Morré, D. J., and Crane, F. L. (1990), inOxidoreduction at the Plasma Membrane: Relation to Growth and Transport. I. Animals (Crane, F. L., Morré, D. J., and Löw, H., eds.). CRC Press, Boca Raton, Florida, pp. 67–84.
Morré, D. J., and Morré, D. M. (1989).Bio Techniques 7, 946–958.
Morré, D. J., Brightman, A., Wang, J., Barr, R., and Crane, F. L. (1988), inProc. NATO Advanced Research Workshop. Plasma Membrane Oxidoreductases in Control of Plant and Animal Growth (Crane, F. L., Löw, H., and Morré, D. L. eds.), Alan R. Liss. New York. pp. 45–55.
Morré, D. J., Crane, F. L., Eriksson, L. C., Löv, H., and Morré, D. M. (1991).Biochim. Biophys. Acta 1057, 140–156.
Morré, D. J., Morré, D. M., Paulik, M., Balova, A., Broome, A.-M., Pirisi, L., and Creek, K. E. (1992).Biochim. Biophys. Acta 1134, 217–222.
Morré, D. J., Davidson, M., Geilen, C., Lawrence, J., Flesher, G., Crowe, R., and Crane, F. L. (1993).Biochem. J. 292, 647–653.
Navas, P., Nowack, D. D., and Morré, D. J. (1989).Cancer Res. 49, 2146–2147.
Pavo, I., and Fahrenholz, F. (1990).FEBS Lett. 272, 205–208.
Sidhu, A., Kassis, S., Kebabian, J., and Fishman, P. H. (1986).Biochemistry 25, 6695–6701.
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, R. H., Provenzano, E. K., Fujimoto, E. K., Gocke, N. M., Oison, B. J., and Klenk, D. C. (1985).Anal. Biochem. 100, 76–85.
Suen, E. T., Stefanini, E., and Clement-Cormier, Y. C. (1980).Biochem. Biophys. Res. Commun. 91, 953–960.
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Morré, D.J., Morré, D.M. Differential response of the NADH oxidase of plasma membranes of rat liver and hepatoma and HeLa cells to thiol reagents. J Bioenerg Biomembr 27, 137–144 (1995). https://doi.org/10.1007/BF02110341
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DOI: https://doi.org/10.1007/BF02110341