Skip to main content
SpringerLink
Log in

Die Wirkung von PGE2 und PGF2α auf zirkuläre und longitudinale Muskelstreifen verschiedener Uterussegmente des Meerschweinchens in vitro

  • Published:
Archives of gynecology Aims and scope Submit manuscript

Summary

The effects of PGE2 and PGF2α on corpus uteri and cervix strips of guinea pigs were studied in vitro. Myometrium strips developed maximal contraction activity at concentrations of 10−7 M PGE2 and 5 × 10−7 M PGF2α in the organ bath, respectively. As for corpus uteri myometrium strips, longitudinal (extern) and circular (intern) layers of the organ wall showed comparable Prostaglandin-induced contractions. On the other hand, longitudinal and circular strips from cervix uteri tissue showed adverse Prostaglandin effects: PGE2 relaxed the cervix strips by lowering the muscle tone, the contraction frequency and a complete stop of contractions within most experiments lasting for an average time of 15 min. PGF2α induced strong contractions of the same kind like corpus uteri strips did. The results indicate a role of PGE2 in the opening of the cervix, whereas PGF2α possibly plays a role in the involution of the uterus post partum.

Zusammenfassung

Mit in vitro-Versuchen wurde die Wirkung von PGE2 und PGF2α auf das Corpus uteri und die Zervix des Meerschweinchens 3–4 Tage nach dem Werfen getestet. Bei Konzentrationen von 10−7 M PGE2 bzw. 5 × 10−7 M PGF2α im Gewebebad wurden die Myometriumstreifen zu maximaler Kontraktionstätigkeit stimuliert.

An den Segmenten des Corpus uteri zeigten longitudinale (äußere) und zirkuläre (innere) Myometriumschichten für beide Prostaglandine Kontraktionen, die sich lediglich in der Dauer der initialen Tonuserhöhung unterschieden.

Im Gegensatz dazu konnten an Zervixstreifen konträre Effekte dargestellt werden: PGE2 relaxierte die Zervixstreifen unter Verminderung der Kontraktionsfrequenz, dem Absinken des Muskeltonus und in den meisten Fällen einem völligen Sistieren der Kontraktionen über ca. 15 min. PGF2α dagegen kontrahierte alle Zervixstreifen in vergleichbarer Weise wie die Corpus uteri-Streifen.

Die Befunde deuten darauf hin, daß PGE2 eine Rolle bei der Zervixeröffnung spielt und PGF2α moglicherweise die Involution des Uterus post partum begünstigt.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  1. Aiken, J. W.: Aspirin and Indomethacin prolong parturition in rats: Evidence that prostaglandins contribute to expulsion of foetus. Nature240, 21 (1972)

    PubMed  Google Scholar 

  2. Bennett, A., Fleshier, B.: Prostaglandins and the gastrointestinal tract. Gastroenterology59, 790 (1970)

    PubMed  Google Scholar 

  3. Csapo, A. I.: Prostaglandins and the initiation of labor. Prostaglandins12, 149 (1976)

    PubMed  Google Scholar 

  4. Conrad, J. T., Ueland, K.: Reduction of stretch modulus of human cervical tissue by prostaglandin E2. Am. J. Obstet. Gynecol.126, 218 (1976)

    PubMed  Google Scholar 

  5. Cooper, G., Schiller, A. L.: Anatomy of the guinea pig, p. 339. Cambridge, Mass.: Harvard Univ. Press 1975

    Google Scholar 

  6. Coutinho, E. M., Darze, E.: Spontaneous contractility and the response of the human cervix to prostaglandin E2 and F2α during the menstrual cycle. Am. J. Obstet. Gynecol.126, 224 (1976)

    PubMed  Google Scholar 

  7. Csapo, A. I.: Effects of progesterone, PGF2α and it's analogue ICI 81 008 on the excitablitiy and threshold of the uterus. Am. J. Obstet. Gynecol.124, 367 (1976)

    PubMed  Google Scholar 

  8. Ehrenpreis, S., Greenberg, J., Comaty, J. E.: Block of electrically induced contractions of guinea pig longitudinal muscle by prostaglandin synthetase and receptor inhibitors. Eur. J. Pharmacol.39, 331 (1976)

    PubMed  Google Scholar 

  9. Eley, K.-G., Bennett, A., Stockley, H. L.: The effects of prostaglandins E1, E2, and F1α on guinea pig ileum and colonic peristalsis. J. Pharm. Pharmacol.29, 276 (1977)

    PubMed  Google Scholar 

  10. Eliasson, V. R., Posse, N.: The effect of prostaglandin on the non pregnant uterus in vivo. Acta Obstet. Gynecol. Scand.39, 112 (1960)

    PubMed  Google Scholar 

  11. Ferreira, S. H., Herman, A. G., Vane, J. R.: Prostaglandin production by rabbit isolated jejunum and it's relationship to the inherent tone of the preparation. Br. J. Pharmacol.56, 469 (1976)

    Google Scholar 

  12. Fleshler, B., Bennett, A.: Response of human, guinea pig and rat colonic muscle to prostaglandins. J. Lab. Clin. Med.74, 872 (1969)

    Google Scholar 

  13. Higgins, Ch. B., Braunwald, E.: The prostaglandins, biological, physiological and clinical considerations. Am. J. Med.53, 92 (1972)

    PubMed  Google Scholar 

  14. Horton, E. W.: Monographs on endocrinology, Vol. 7. Berlin, Heidelberg, New York: Springer 1972

    Google Scholar 

  15. Jung, H.: Physiologie der Wehentätigkeit. Pathologie der Wehentätigkeit — Uterine Dystokie. Gynakologe7, 59 (1974)

    Google Scholar 

  16. Karim, S. M. M.: The effect of ethylalcohol on prostaglandins E2 and F2α induced uterine activity in pregnant women. Br. J. Obstet. Gynecol.78, 251 (1971)

    Google Scholar 

  17. Karim, S. M. M.: Prostaglandins and reproduction. 6th. Internations Congress Pharmacol. Helsinki, Vol. 5, p. 3, 1976

  18. Keirse, M. J. N. C., Turnbull, A. C.: In vitro metabolism of prostaglandins in tissues from the pregnant human uterus. J. Endocrinol.64, 18 (1975)

    Google Scholar 

  19. Kuriyama, H., Suzuki, H.: Effects of Prostaglandin E2 and Oxytocin on the electrical activity of hormone treated and pregnant rat myometria. J. Physiol.260, 335 (1976)

    PubMed  Google Scholar 

  20. Liggins, G. C., Fairclough, R. J., Greives, S. A., Kendall, J. Z., Knox, B. S.: The mechanism of initiation of parturition in the ewe. Recent Prog. Horm. Res.29, 111 (1973)

    PubMed  Google Scholar 

  21. Laties, A. M., Neufeld, A. H., Vegge, T., Sears, M. L.: Differential reactivity of rabbit iris and ciliary muscle to topically applied Prostaglandin E2. Arch. Ophtalmol.94, 1966 (1976)

    Google Scholar 

  22. Main, I. H. M.: The inhibitory actions of prostaglandins on respiratory smooth muscle. Br. J. Pharmacol.22, 511 (1964)

    Google Scholar 

  23. Naaktgeboren, C. N., Slijper, E. J.: Biologie der Geburt. Hamburg: Paul Parey 1970

    Google Scholar 

  24. Najak, Z., Hillier, K., Karim, S. M. M.: The action of prostaglandins on the human isolated nonpregnant cervix. Br. J. Obstet. Gynaecol.77, 701 (1970)

    Google Scholar 

  25. Reiner, O., Marshall, J. M.: Action of PGF2α on the uterus of the pregnant rat. Naunyn-Schmiedebergs Arch. Pharmacol.293, 243 (1976)

    Google Scholar 

  26. Rubin, B., Grigas, E. O.: Prostaglandin antagonists: Synthesis and smooth muscle activity. Nature223, 208 (1969)

    PubMed  Google Scholar 

  27. Sandberg, F., Ingelman-Sundberg, A., Ryden, G.: The effect of Prostaglandin E2 and E3 on the human uterus and fallopian tubes in vitro. Acta Obstet. Gynecol. Scand.43, 95 (1964)

    PubMed  Google Scholar 

  28. Sandberg, F., Ingelman-Sundberg, A., Ryden, G.: The specific effect of prostaglandin on different parts of the human fallopian tube. Br. J. Obstet. Gynecol.70, 130 (1963)

    Google Scholar 

  29. Schröder, R.: Die weiblichen Genitalorgane. Handbuch der mikroskopischen Anatomie des Menschen. Bd. VII/1 (1930) — Gynäkologie. Berlin, Göttingen, Heidelberg: Springer 1947

    Google Scholar 

  30. Van Alphen, G. W. H. M., Angel, M. A.: Activity of prostaglandin E, F, A and B on sphincter, dilatator and ciliary muscle preparation of the cat eye. Prostaglandins9, 157 (1975)

    PubMed  Google Scholar 

  31. Von Euler, U.: Zur Kenntnis er pharmakologischen Wirkungen von Nativsekreten und Extrakten akzessorischer Geschlechtsdrüsen. Naunyn-Schmiedebergs Arch. Pharmacol.175, 78 (1934)

    Google Scholar 

  32. Wagner, J. E., Manning, P. J.: The biology of the guinea pig. New York: Academic Press 1976

    Google Scholar 

  33. Wiquist, N., Roth-Brandel, U., Bygdeman, M.: The effect of prostaglandin E1 and F2α on the nonpregnant human uterus in vitro. Int. J. Obstet. Gynec.8, 165 (1970)

    Google Scholar 

  34. Yamaguchi, T., Hitzig, B., Coburn, R. F.: Endogeneous prostaglandin in guinea pig taenia coli. Am. J. Physiol.230, 149 (1976)

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Frauenklinik der Medizinischen Hochschule Hannover, Abt. I (Prof. Dr. J. Schneider), D-3000, Hannover 61, Bundesrepublik Deutschland

    M. Cornely

  2. Institut für Pharmakologie und Toxikologie der Medizinischen Hochschule Hannover, Abt. III, Biochemische Pharmakologie (Prof. Dr. H. Scholz), D-3000, Hannover 61, Bundesrepublik Deutschland

    I. Hackbarth

Authors
  1. M. Cornely
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Hackbarth
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cornely, M., Hackbarth, I. Die Wirkung von PGE2 und PGF2α auf zirkuläre und longitudinale Muskelstreifen verschiedener Uterussegmente des Meerschweinchens in vitro. Arch. Gynecol. 227, 83–95 (1979). https://doi.org/10.1007/BF02108630

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02108630

Key words

Schlüsselwörter

Search

Navigation