Summary
The physical localization of sequences homologous to three cloned genes was determined by in situ hybridization to metaphase chromosomes. Previous work had assigned the skeletal myosin heavy chain gene cluster (Myh), the functional locus for the cellular tumor antigen p53 (Trp53-1), and the cellular homologue of the viral erb-B oncogene (Erbb) toMus musculus chromosome 11 (MMU11). Our results provide regional assignments ofMyh andTrp53-1 to chromosome bands B2→C, and ofErbb to bands A1→A4. Taken together with in situ mapping of three other loci on MMU 11 (Hox-2 homeobox-containing gene cluster, theSparc protein, and theColla-1 collagen gene), which have been reported elsewhere, these data allowed us to construct a physical map of MMU11 and to compare it with the linkage map of this chromosome. The map positions of the homologous genes on human chromosomes suggest evolutionary relationships of distinct regions of MMU11 with six different human chromosome arms: 1p, 5q, 7p, 16p, 17p, and 17q. The delineation of conserved chromosome regions has important implications for the understanding of karyotype evolution in mammalian species and for the development of animal models of human genetic diseases.
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Barlow DP, Bucán M, Lehrach H, Hogan BLM, Gough NM (1987) Close genetic and physical linkage between the murine haemopoietic growth factor genes GM-CSF and MULTI-CSF (IL3). EMBO J (in press)
Benchimol S, Lamb P, Crawford LV, Sheer D, Shows TB, Bruns GAP, Peacock J (1985) Transformation associated p53 protein is encoded by a gene on human chromosome 17. Somatic Cell Mol Genet 11:505–509
Cannizzaro LA, Emanuel, BS, Cho KWY, Weinmann R (1986) The gene encoding the large subunit of human RNA polymerase II is located on the short arm of chromosome 17. Am J Hum Genet 38:812–818
Cattanach BM, Kirk M (1985) Differential activity of maternally and paternally derived chromosome regions in mice. Nature 315:496–498
Coussens L, Yang-Feng TL, Liao Y-C, Chen E, Gray A, McGrath J, Seeburg PH, Libermann TA, Schlessinger J, Francke U, Levinson A, Ullrich A (1985) Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location withneu oncogene. Science 230:1132–1139
Czosnek H, Nudel U, Shani M, Barker PE, Pravtcheva DD, Ruddle FH, Yaffe D (1982) The genes coding for the muscle contractile proteins, myosin heavy chain, myosin light chain 2, and skeletal muscle actin are located on three different mouse chromosomes. EMBO J 1:1299–1305
Czosnek H, Bienz B, Givol D, Zakut-Houri R, Pravtcheva DD, Ruddle FH, Oren M (1974) The gene and pseudogene for mouse p53 cellular tumor antigen are located on different chromosomes. Mol Cell Biol 4:1638–1640
Davisson MT, Roderick TH (1985) Mouse Newsl 73:6–8
Downward J, Yarden Y, Mayes E, Scrace G, Totty N, Stockwell P, Ullrich A, Schlessinger J, Waterfield MD (1984) Close similarity of epidermal growth factor receptor andv-erb-B oncogene protein sequences. Nature 307:521–527
Edwards YH, Parkar M, Povey S, West LF, Parrington JM, Solomon E (1985) Human myosin heavy chain genes assigned to chromosome 17 using a human cDNA clone as probe. Ann Hum Genet 49:101–109
Elsevier SM, Kucherlapati RS, Nichols EA, Creagan RP, Giles RE, Ruddle FH, Willecke K, McDougall JK (1974) Assignment of the gene for galactokinase to human chromosome 17 and its regional localization to band q21→22. Nature 251: 633–635
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Feinberg AP, Vogelstein B (1984) Addendum to A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity (Anal Biochem 132:6–13). Anal Biochem 137:266–267
Francke U, Lalley PA, Moss W, Ivy J, Minna JD (1977) Gene mapping inMus musculus by interspecific cell hybridization: assignment of the genes for tripeptidase-1 to chromosome 10, dipeptidase-2 to chromosome 18, acid phosphatase-1 to chromosome 12 and adenylate kinase-1 to chromosome 2. Cytogenet Cell Genet 19:57–84
Francke U, Yang-Feng TL, Brissenden JE, Ullrich A (1987) Chromosomal mapping of genes involved in growth control. In: Molecular biology ofHomo sapiens. Cold Spring Harbor Symp Quant Biol 51. New York (in press)
Gough NM, Gough J, Metcalf D, Kelso A, Grail D, Nicola NA, Burgess AW, Dunn AR (1984) Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor. Nature 309:763–767
Harper ME, Saunders GF (1981) Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization. Chromosoma 83:431–439
Huerre C, Junien C, Weil D Chu M-L, Morabito M, Nguyen, Van Cong, Myers JC, Foubert C, Gross M-S, Prockop DJ, Boue A, Kaplan J-C, de la Chapelle A, Ramirez F (1982) Human type I procollagen genes are located on different chromosomes. Proc Natl Acad Sci USA 79:6627–6630
Human Gene Mapping 8 (1985) Eighth international workshop on human gene mapping. Cytogenet Cell Genet 40:1–823
Human Gene Mapping Library (1986) Regional localizations of genes and genetic markers to chromosomes and subregions of chromosomes. Howard Hughes Medical Institute, New Haven, CT
Joyner AL, Lebo RV, Kan YW, Tjian R, Cox DR, Martin GR (1985) Comparative chromosome mapping of a conserved homoeobox region in mouse and human. Nature 314:173–175
Killary AM, Fournier REK (1984) A genetic analysis of extinction:trans-dominant loci regulate expression of liver-specific traits in hepatoma hybrid cells. Cell 38:523–534
Kozak CA, Ruddle FH (1977) Assignment of the genes for thymidine kinase and galactokinase toMus musculus chromosome 11 and the preferential segregation of this chromosome in Chinese hamster/mouse somatic cell hybrids. Somatic Cell Genet 3:121–133
Kucherlapati R, McDougall JK, Ruddle FH (1974) Regional localization of the human genes for thymidine kinase, lactate dehydrogenase-A and esterase A4. Cytogenet Cell Genet 13: 108–110
Lalley PA, McKusick VA (1985) Report of the committee on comparative mapping. In: Human gene mapping 8. Cytogenet Cell Genet 40:536–566
Lamb P, Crawford L (1986) Characterization of the human p53 gene. Mol Cell Biol, 6:1379–1385
Le Beau MM, Westbrook CA, Diaz MO, Rowley JD, Oren M (1985) Translocation of the p53 gene in t(15;17) in acute promyelocytic leukaemia. Nature 316:826–828
Le Beau MM, Westbrook CA, Diaz MO, Larson RA, Rowley JD, Gasson JC, Golde DW, Sherr CJ (1986) Evidence for the involvement of GM-CSF and FMS in the deletion (5q) in myeloid disorders. Science 231:984–987
Leinwand LA, Fournier REK, Nadal-Ginard B, Shows TB (1983) Multigene family for sarcomeric myosin heavy chain in mouse and human DNA: localization on a single chromosome. Science 221:766–769
Lin CC, Draper PN, De Braekeleer M (1985) High-resolution chromosomal localization of the β-gene of the human β-globin gene complex by in situ hybridization. Cytogenet Cell Genet 39:269–274
Lindgren V, Bernstein LB, Weiner AM, Francke U (1985a) Human U1 small nuclear RNA pseudogenes do not map to the site of the U1 genes in 1p36 but are clustered in 1q12→q22. Mol Cell Biol 5:2172–2180
Lindgren V, Ares Jr M, Weiner AM, Francke U (1985b) Human genes for U2 small nuclear RNA map to a major adenovirus 12 modification site on chromosome 17. Nature 314:115–116
Mason IJ, Taylor A, Williams JG, Sage H, Hogan BLM (1986a) Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell “culture shock” glycoprotein of Mr 43000. EMBO J 5:1464–1472
Mason IJ, Murphy D, Münke M, Francke U, Elliott RW, Hogan BLM (1986b) Developmental and transformation-sensitive expression of theSparc gene on mouse chromosome 11. EMBO J 5:1831–1837
McBreen P, Orkwiszewski KG, Chern CJ, Mellman WJ, Croce CM (1977) Synteny of the genes for thymidine kinase and galactokinase in the mouse and their assignment to mouse chromosome 11. Cytogenet Cell Genet 19:7–13
McBride OW, Merry D, Givol D (1986) The gene for human p53 cellular tumor antigen is located on chromosome 17 short arm (17p13). Proc Natl Acad Sci USA 83:130–134
Michael SK, Hilgers J, Kozak C, Whitney III JB, Howard EF (1986) Characterization and mapping of DNA sequence homologous to mouse U1a1 snRNA: localization on chromosome 11 near theDlb-1 andRe loci. Somatic Cell Mol Genet 12:215–223
Miller C, Mohandas T, Wolf D, Prokocimer M, Rotter V, Koeffler HP (1986) Human p53 gene localized to short arm of chromosome 17. Nature 319:783–784
Münke M, Lindgren V, de Martinville B, Francke U (1984) Comparative analysis of mouse-human hybrids with rearranged chromosomes 1 by in situ hybridization and Southern blotting: high-resolution mapping of NRAS, NGFB, and AMY on chromosome 1. Somatic Cell Mol Genet 10:589–599
Münke M, Harbers K, Jaenisch R, Francke U (1986a) Chromosomal mapping of four different integration sites of Moloney murine leukemia virus including the locus for α1(I) collagen in mouse. Cytogenet Cell Genet 43:140–149
Münke M, Cox DR, Jackson IJ, Hogan BLM, Francke U (1986b) The murineHox-2 cluster of homeo box containing genes maps distal on chromosome 11 near the tail-short (Ts) locus. Cytogenet Cell Genet 42:236–240
Münke M, Hogan BLM, Jackson IJ, Francke U (1986c) Regional mapping of genes on mouse chromosome 11 and comparison to the homologous loci in man. 7th Int Congr Hum Genet, Abstr II:605
Naylor SL, Zabel BU, Manser T, Gesteland R, Sakaguchi AY (1984) Localization of human U1 small nuclear RNA genes to band p36.3 of chromosome 1 by in situ hybridization. Somatic Cell Mol Genet 10:307–313
Nesbitt M, Francke U (1973) A system of nomenclature for band patterns of mouse chromosomes. Chromosoma 41:145–158
Nojima H, Kornberg RD (1983) Genes and pseudogenes for mouse U1 and U2 small nuclear RNAs. J Biol Chem 258: 8151–8155
Oren M (1985) The p53 cellular tumor antigen: gene structure, expression and protein properties. Biochim Biophys Acta 823: 67–78
Oren M, Levine AJ (1983) Molecular cloning of a cDNA specific for the murine p53 cellular tumor antigen. Proc Natl Acad Sci USA 80:56–59
Pravtcheva D, Rabin M, Bartolomei M, Corden J, Ruddle FH (1986) Chromosomal assignment of gene encoding the largest subunit of RNA polymerase II in the mouse. Somatic Cell Mol Genet 12:523–528
Rabin M, Hart CP, Ferguson-Smith A, McGinnis W, Levine M, Ruddle FH (1985) Two homoeobox loci mapped in evolutionarily related mouse and human chromosomes. Nature 314:175–178
Rappold GA, Vosberg H-P (1983) Chromosomal localization of a human myosin heavy-chain gene by in situ hybridization. Hum Genet 65:195–197
Rotter V, Wolf D, Pravtcheva D, Ruddle FH (1984) Chromosomal assignment of the murine gene encoding the transformation-related protein p53. Mol Cell Biol 4:383–385
Saez L, Leinwand LA (1986) Characterization of diverse forms of myosin heavy chain expressed in adult human skeletal muscle. Nucleic Acids Res 14:2951–2968
Sawyer JR, Hozier JC (1986) High resolution of mouse chromosomes: banding conservation between man and mouse. Science 232:1632–1635
Schnieke A, Harbers K, Jaenisch R (1983) Embryonic lethal mutation in mice induced by retrovirus insertion into the alpha(I) collagen gene. Nature 304:315–320
Silver J, Whitney III JB, Kozak C, Hollis G, Kirsch I (1985)Erbb is linked to the alpha-globin locus on mouse chromosome 11. Mol Cell Biol 5:1784–1786
Solomon E, Hiorns L, Sheer D, Rowe D (1984) Confirmation that the type I collagen gene on chromosome 17 is COL1A1 using a human genomic probe. Ann Hum Genet 48:39–42
Spurr NK, Solomon E, Jansson M, Sheer D, Goodfellow PN, Bodmer WF, Vennstrom B (1984) Chromosomal localisation of the human homologues to the oncogeneserbA and B. EMBO J 3:159–163
Taylor BA (1978) Recombinant inbred strains: use in gene mapping. In: Morse III HC (ed) Origins of inbred mice. Academic Press, New York San Francisco London, p 423
Ullrich A, Coussens L, Hayflick, JS, Dull TJ, Gray A, Tam AW, Lee J, Yarden Y, Libermann TA, Schlessinger J, Downward J, Mayes ELV, Whittle N, Waterfield MD, Seeburg PH (1984) Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. Nature 309:418–425
Weydert A, Daubas P, Lazaridis I, Barton P, Garner I, Leader DP, Bonhomme F, Catalan J, Simon D, Guenet JL, Gros F, Buckingham ME (1985) Genes for skeletal muscle myosin heavy chains are clustered and are not located on the same mouse chromosome as a cardiac myosin heavy chain gene. Proc Natl Acad Sci USA 82:7183–7187
Wilson DE, Swallow DM, Povey S (1986) Assignment of the human gene for uridine 5′-monophosphate phosphohydrolase (UMPH2) to the long arm of chromosome 17. Ann Hum Genet 50:223–227
Wilson DE, Woodard D, Sandler A, Erickson J, Gurney A (1987) Provisional assignment of the gene for uridine monophosphatase-2 (Umph-2) to mouse chromosome 11. Biochem Genet 25:1–6
Zabel BU, Fournier REK, Lalley PA, Naylor SL, Sakaguchi AY (1984) Cellular homologs of the avian erythroblastosis viruserb-A anderb-B genes are syntenic in mouse but asyntenic in man. Proc Natl Acad Sci USA 81:4874–4878
Zakut-Houri R, Oren M, Bienz B, Lavie V, Hazum S, Givol D (1983) A single gene and a pseudogene for the cellular tumour antigen p53. Nature 306:594–597
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Münke, M., Francke, U. The physical map ofMus musculus chromosome 11 reveals evolutionary relationship with different syntenic groups of genes inHomo sapiens . J Mol Evol 25, 134–140 (1987). https://doi.org/10.1007/BF02101755
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DOI: https://doi.org/10.1007/BF02101755