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Phylogenetic relationships of the green algeVolvox carteri deduced from small-subunit ribosomal RNA comparisons

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Summary

The 1788-nucleotide sequence of the small-subunit ribosomal RNA (srRNA) coding region from the chlorophyteVolvox carteri was determined. The secondary structure bears features typical of the universal model of srRNA, including about 40 helices and a division into four domains. Phylogenetic relationships to 17 other eukaryotes, including two other chlorophytes, were explored by comparing srRNA sequences. Similarity values and the inspection of phylogenetic trees derived by distance matrix methods revealed a close relationship betweenV. carteri andChlamydomonas reinhardtii. The results are consistent with the view that these Volvocales, and the third green alga,Nanochlorum eucaryotum, are more closely related to higher plants than to any other major eukaryotic group, but constitute a distinct lineage that has long been separated from the line leading to the higher plants.

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References

  • Benton WD, Davis RS (1977) Screening λgt recombinant clones by hybridization to single plaques in situ. Science 196:180–183

    PubMed  Google Scholar 

  • Bold HC, Wynne MJ (1985) Introduction to the algae, ed 2. Prentice-Hall, Englewood NJ

    Google Scholar 

  • Dams E, Hendriks L, Van de Peer Y, Neefs JM, Smits G, Vandenbempt I, DeWachter R (1988) Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res 7:87–173

    Google Scholar 

  • Eckenrode V, Arnold J, Meagher RB (1985) Comparison of the nucleotide sequence of soybean 18S rRNA with the sequences of other small-subunit rRNAs. J Mol Evol 21:259–269

    Google Scholar 

  • Elwood HJ, Olsen GJ, Sogin ML (1985) The small-subunit ribosomal RNA gene sequence from the hypotrichous ciliateOxytricha nova andStylonychia pustulata. Mol Biol Evol 2:399–410

    PubMed  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  PubMed  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Google Scholar 

  • Fitch WM, Margoliash E (1967) Construction of phylogenetic trees. Science 155:279–284

    PubMed  Google Scholar 

  • Fox GE, Stackebrandt E, Hespell RB, Gibson J, Dyer TA, Wolfe RS, Balch WE, Tanner R, Magrum L, Zablen LB, Blakemore R, Gupta R, Bonen L, Lewis BJ, Stahl DA, Luehrsen KR, Chen KN, Woese CR (1980) The phylogeny of prokaryotes. Science 209:457–463

    PubMed  Google Scholar 

  • Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885

    PubMed  Google Scholar 

  • Gunderson JH, Elwood H, Ingold A, Kindle K, Sogin ML (1987) Phylogenetic relationships between chlorophytes, chrysophytes and oomycetes. Proc Natl Acad Sci USA 84:5823–5827

    PubMed  Google Scholar 

  • Gutell RR, Weiser B, Woese CR, Noller HF (1985) Comparative anatomy of 16S-like ribosomal RNA. Prog Nucleic Acid Res Mol Biol 32:155–216

    PubMed  Google Scholar 

  • Harper JF, Mages W (1988) Organization and structure ofVolvox β-tubulin genes. Mol Gen Genet 213:315–324

    Article  PubMed  Google Scholar 

  • Heinrich P (1986) Guidelines for quick and simple plasmid sequencing. Boehringer Monograph, Mannheim

  • Herzog M, Maroteaux L (1986) Dinoflagellate 17S rRNA sequence inferred from the gene sequence: evolutionary implications. Proc Natl Acad Sci USA 83:8644–8648

    Google Scholar 

  • Hori H, Osawa S (1979) Evolutioanary change in 5S RNA secondary structure and a phylogenetic tree of 54 5S RNA species. Proc Natl Acad Sci USA 76:381–385

    PubMed  Google Scholar 

  • Huysmans E, DeWachter R (1986) Complication of small ribosomal subunit RNA sequences. Nucleic Acids Res 14:r77-r118

    Google Scholar 

  • Johnson RA, Walseth TF (1979) The enzymatic preparation of [α32P]nucleoside-triphosphates, cyclic [32P]AMP and cyclic [32P]GMP. Biochim Biophys Acta 562:11–23

    PubMed  Google Scholar 

  • Kirk DL, Harper JF (1986) Genetic, biochemical, and molecular approaches toVolvox development and evolution. Int Rev Cytol 99:217–293

    PubMed  Google Scholar 

  • Kochert G (1973) Colony differentiation in green algae. In: Coward SJ (ed) Developmental regulation. Academic Press, New York, pp 155–167

    Google Scholar 

  • Leffers H, Kjems J, Ostergaard L, Larsen N, Garrett RA (1987) Evolutionary relationships amongst the archaebacteria. A comparative study of 23S ribosomal RNAs of sulfur dependent extreme thermophile, an extreme halophile and a thermophile methanogene. J Mol Biol 195:43–61

    PubMed  Google Scholar 

  • Mages W, Salbaum JM, Harper JF, Schmitt R (1988) Organization and structure ofVolvox α-tubulin genes. Mol Gen Genet 213:449–458

    Article  PubMed  Google Scholar 

  • Maniatis T, Fritsch EF, Sambrock J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor NY

    Google Scholar 

  • Mankin AS, Skryabin KG, Rubtsov PM (1986) Identification of ten additional nucleotides in the primary structure of yeast 18S rRNA. Gene 44:143–145

    Article  PubMed  Google Scholar 

  • Matteucci MD, Caruthers MH (1981) Synthesis of deoxyoligonucleotides on a polymer support. J Am Chem Soc 103:3185–3191

    Article  Google Scholar 

  • Maxam AM, Gilbert W (1980) Sequencing end-labelled DNA with base-specific chemical cleavages. Meth Enzymol 65:499–559

    PubMed  Google Scholar 

  • McCarrol R, Olsen GJ, Stahl YD, Woese CR, Sogin ML (1983) Nucleotide sequence of theDictyostelium discoideum smallsubunit ribosomal ribonucleic acid inferred from the gene sequence: evolutionary implications. Biochemistry 22:5858–5868

    Article  Google Scholar 

  • Messing J, Carlson J, Hagen G, Rubenstein J, Oleson A (1984) Cloning and sequencing of the ribosomal RNA genes in maize: the 17S region. DNA 3:31–40

    PubMed  Google Scholar 

  • Müller K, Schmitt R (1988) Histone genes ofVolvox carteri: DNA sequence and organization of two H3-H4 gene loci. Nucleic Acids Res 16:4121–4136

    PubMed  Google Scholar 

  • Ochman H, Wilson AC (1987) Evolution in bacteria: evidence for a universal substitution rate in cellular genomes. J Mol Evol 26:74–86

    PubMed  Google Scholar 

  • Raven PH, Evert RF, Eichorn SE (1986) Biology of plants, ed 4. Worth, New York.

    Google Scholar 

  • Raynal F, Michot B, Bachellerie J-P (1984) Complete nucleotide sequence of mouse 18S rRNA gene: comparison with other available homologs. FEBS Lett 167:263–268

    Article  PubMed  Google Scholar 

  • Rubtsov PM, Musakhanov MM, Zakhayev VM, Krayev AS, Skryabin KG, Bayev AA (1980) The structure of the yeast ribosomal RNA genes. The complete sequence of the 18S ribosomal RNA gene fromSaccharomyces cerevisiae. Nucleic Acids Res 8:5779–5794

    PubMed  Google Scholar 

  • Salim M, Maden BEH (1981) Nucleotide sequence ofXenopus laevis 18S ribosomal RNA inferred from gene sequence. Nature 291:205–208

    Article  PubMed  Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain termination inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    PubMed  Google Scholar 

  • Sargent M, Zahn R, Walters B, Gupta R, Kaine B (1988) Nucleotide sequence of the 18S rDNA from microalgaNanochlorum eucaryotum. Nucleic Acids Res 16:4156

    PubMed  Google Scholar 

  • Sogin ML, Elwood HJ (1986) Primary structure of theParamecium tetraurelia small-subunit rRNA coding region: phylogenetic relationships within ciliophora. J Mol Evol 23:53–60

    PubMed  Google Scholar 

  • Sogin ML, Elwood HJ, Gunderson JH (1986a) Evolutionary diversity of eukaryotic small-subunit rRNA genes. Proc Natl Acad Sci USA 83:1383–1387

    PubMed  Google Scholar 

  • Sogin ML, Swanton MJ, Gunderson JH (1986a) Evolutionary the small-subunit ribosomal RNA gene from the hypotrichous ciliateEuplotes aediculatus. J Protozool 33:26–29

    PubMed  Google Scholar 

  • Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    PubMed  Google Scholar 

  • Spangler EA, Blackburn EH (1985) The nucleotide sequence of the 17S ribosomal RNA gene ofTetrahymena thermophila and the identification of point mutations resulting in resistance to the antibiotics paromomycin and hygromycin. J Biol Chem 160:6334–6340

    Google Scholar 

  • Starr RC (1969) Structure, reproduction and differentiation inVolvox carteri f.nagariensis Iyengar, strains HK10 and HK9. Arch Protistenkd 111:204–222

    Google Scholar 

  • Takaiwa F, Oono K, Sugiura M (1984) The complete nucleotide sequence of a rice 17S rRNA gene. Nucleic Acids Res 12:5441–5448

    PubMed  Google Scholar 

  • Vieira J, Messing J (1982) The pUC plasmids an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268

    Article  PubMed  Google Scholar 

  • Vossbrinck CR, Maddox JV, Friedman S, Debrunner-Vossbrick BA, Woese CR (1987) Ribosomal RNA sequence suggests Microsporidia are extremely ancient eukaryotes. Nature 326:411–414

    Article  PubMed  Google Scholar 

  • Woese CR (1987) Bacterial evolution. Microbiol Rev 51:221–271

    PubMed  Google Scholar 

  • Woese CR, Gutell R, Gupta R, Noller HF (1983) Detailed analysis of the 16S-like ribosomal ribonucleic acids. Microbiol Rev 47:621–669

    PubMed  Google Scholar 

  • Yates F, Kochert G (1976) Nucleic acid and protein differences inVolvox carteri cell types. Cytobios 15:7–21

    PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Lehrstuhl für Genetik, Universität Regensburg, D-8400, Regensburg, Federal Republic of Germany

    Helmut Rausch & Rüdiger Schmitt

  2. Biostructural Chemistry, Institute of Chemistry, Aarhus University, DK-8000, Aarhus, Denmark

    Niels Larsen

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  1. Helmut Rausch
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  2. Niels Larsen
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  3. Rüdiger Schmitt
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Rausch, H., Larsen, N. & Schmitt, R. Phylogenetic relationships of the green algeVolvox carteri deduced from small-subunit ribosomal RNA comparisons. J Mol Evol 29, 255–265 (1989). https://doi.org/10.1007/BF02100209

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  • DOI: https://doi.org/10.1007/BF02100209

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