Abstract
Rhizobium trifolii organisms associated with eleven species ofTrifolium were isolated and studied by means of antigen-antibody agglutination and immune diffusion techniques. Ten serologic types were differentiated from the eleven species of nativeTrifolium.
Certain serologic types of rhizobia were widely distributed among the native species ofTrifolium, whereas other rhizobial types were found only on one or two species. The distribution pattern appears to be independent of the proportion of each type present in the soil population. This may indicate specificity of selection between a host legume and serologic types of rhizobia.
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References
Annear, D. I. 1956. Freeze drying. III. The preservation of bacteria. Lab. Pract.1956 102–105.
Bergersen, F. J. 1961. The growth of rhizobium in synthetic media. Australian J. Biol. Sci.14 349–360.
Brockwell, J. 1963. Accuracy of a plant-infection technique for counting populations ofRhizobium trifolii. Appl. Microbiol.11 377–383.
Bushnell, O. A. andSarles, W. B. 1939. Investigations upon the antigenic relationships of the root-nodule bacteria of the soybean, cowpea and lupine cross-inoculation groups. J. Bacteriol.38 401–410.
Crowle, A. J. 1960. Interpretation of immunodiffusion tests. Ann. Rev. Microbiol.14 161–176.
Crowle, A. J. 1961. Immunodiffusion. Academic Press, Inc., New York.
Dudman, W. F. 1964. Immune diffusion analysis of the extra-cellular soluble antigens of two strains ofRhizobium meliloti. J. Bacteriol.88 782–794.
Evans, E. E. 1957. Serological methods, p. 199–223.In M. J. Pelczar, [ed.], Manual of microbiological methods. McGraw-Hill, New York.
Graham, P. H. 1963. Antigenic affinities of the root-nodule bacteria of legumes. Antonie van Leeuwenhoek29 281–291.
Holland, A. A. 1964. Clover establishment in Northern California. Calif. Agr.18 (7): 15.
Hughes, D. Q. andVincent, J. M. 1942. Serological studies of root-nodule bacteria. III. Tests of neighbouring strains of the same species. Proc. Linnean Soc. N.S. Wales67 142–152.
Johnson, H. W. andMeans, U. M. 1963. Serological groups ofRhizobium japonicum recovered from nodules of soybeans (Glycine max) in field soils. Agron. J.55 269–271.
Kleczkowski, A. andThornton, H. G. 1944. A serological study of root nodule bacteria from pea and clover inoculation groups. J. Bacteriol.48 661–672.
Koontz, F. P. andFaber, J. E. 1961. Somatic antigens ofRhizobium japonicum. Soil Sci.91 228–232.
Loos, M. A. andLouw, H. A. 1964. A study of the clover root-nodule bacteria in soils of the George District. I. Serological characteristics. S. African J. Agr. Sci.7 135–146.
Nutman, P. S. 1946. Variation within strains of clover nodule bacteria in the size of nodule produced and in the “effectivity” of the symbiosis. J. Bacteriol.51 411–432.
Purchase, H. F., Vincent, J. M. andWaters, L. M. 1951. Serological studies of the rootnodule bacteria. IV. Further analysis of isolates fromTrifolium andMedicago. Proc. Linnean Soc. N.S. Wales76 1–6.
Vincent, J. M. 1941. Serological studies of the root-nodule bacteria. I. Strains ofRhizobium meliloti. Proc. Linnean Soc. N.S. Wales66 145–154.
Vincent, J. M. 1942. Serological studies of the root-nodule bacteria. II. Strains ofRhizobium trifolii. Proc. Linnean Soc. N.S. Wales67 82–86.
Vincent, J. M. andWaters, L. M. 1953. The influence of the host on competition amongst clover root-nodule bacteria. J. Gen. Microbiol.9 357–370.
Vintiková, H., Šrogl, M. andŠkrdleta, V. 1961. A contribution to the serological typization of the rhizobia. Folia Microbiol. (Prague)6 243–249.
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Holland, A.A. Serologic characteristics of certain root-nodule bacteria of legumes. Antonie van Leeuwenhoek 32, 410–418 (1966). https://doi.org/10.1007/BF02097492
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DOI: https://doi.org/10.1007/BF02097492