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Cloning and expression of anAeromonas hydrophila chitinase gene inescherichia coli

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Abstract

The gene encoding an extracellular chitinase fromAeromonas hydrophila has been cloned and expressed inEscherichia coli. Plasmid pJP2512 contained the smallest DNA insert (3.9 kb) producing chitinase. The chitinase gene is transcribed from its own promoter, producing a protein of Mr 96,000. The chitinase open reading frame, an estimated 2.6 kb in length, has been subcloned to a 3.0 kb fragment; however, this fragment does not carry the functional chitinase promoter. InE. coli the chitinase enzyme is unable to transverse the outer membrane, being secreted across the cytoplasmic membrane and accumulating in the periplasmic space.

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  1. Department of Microbiology, University of Queensland, Brisbane, Australia

    Paul E. Roffey & John M. Pemberton

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  1. Paul E. Roffey
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  2. John M. Pemberton
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Roffey, P.E., Pemberton, J.M. Cloning and expression of anAeromonas hydrophila chitinase gene inescherichia coli . Current Microbiology 21, 329–337 (1990). https://doi.org/10.1007/BF02092100

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