Summary
1. The effects of restraint stress on c-fos mRNA expression in the dentate gyrus were investigated byin situ hybridization.
2. Confirming previous findings, c-fos mRNA expression increased after 30 min of forced restraint.
3. This effect was attenuated by a previous i.c.v. injection of the anxiolytic benzodiazepine midazolam (20 nmol/2 µl) or theN-methyl-d-aspartate (NMDA) receptor antagonist 2-amino-7-phosphonoheptanoic acid (AP-7; 5 nmol/2 µl).
4. These results suggest that the dentate gyrus is activated during restraint stress and that this activation may be modulated by benzodiazepine γ-aminobutyric acidA (GABAA) or NMDA receptors.
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Titze-de-Almeida, R., de Oliveira, C.L., Shida, H.W. et al. Midazolam and theN-methyl-d-aspartate (NMDA) receptor antagonist 2-amino-7-phosphonoheptanoic acid (AP-7) attenuate stress-induced expression of c-fos mRNA in the dentate gyrus. Cell Mol Neurobiol 14, 373–380 (1994). https://doi.org/10.1007/BF02088717
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DOI: https://doi.org/10.1007/BF02088717