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Hepatic stimulator substance administration enhances regenerative capacity of hepatocytes in cadmium-pretreated partially hepatectomized rats

  • Liver: Infectious, Inflammatory, And Metabolic Disorders
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Abstract

The liver is of central importance in the metabolism of essential and toxic metals such as cadmium (Cd). Cd pretreatment suppressed the regenerative capacity of hepatocytes, which normally occurs 24 hr after partial hepatectomy, due to the inhibition of the activity of the enzyme thymidine kinase. The effect of hepatic stimulator substance (HSS) administration (10, 20, and 40 mg protein/kg body weight) on hepatocyte proliferation was investigated in Cd-pretreated partially hepatectomized rats. HSS administration partly restored the suppressed hepatocyte DNA biosynthesis in Cd-pretreated partially hepatectomized rats. The hepatocyte mitotic activity and the percentage of proliferating cell nuclear antigen-positive nuclei were in accordance with the liver proliferative status. The administration of HSS did not affect in a statistically significant manner the activity of the enzyme thymidine kinase in Cd-pretreated partially hepatectomized rats. It is suggested that the administration of HSS ameliorates the diminished hepatocyte regenerative response to partial hepatectomy in this model of acute liver injury, due to Cd intoxication.

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Authors and Affiliations

  1. From the Department of Forensic Medicine and Toxicology, University of Athens, 75, M. Asias str., GR 115 27, Goudi, Athens, Greece

    Stamatios E. Theocharis MD, Alexandra P. Margeli PhD, Chara Spiliopoulou MD, PhD, Spyridon Skaltsas MD, PhD, Christos Kittas MD, PhD & Antonios Koutselinis MD, PhD

  2. Department of Histology and Embryology, School of Medicine, University of Athens, 75, M. Asias str., GR 115 27, Goudi, Athens, Greece

    Stamatios E. Theocharis MD, Alexandra P. Margeli PhD, Chara Spiliopoulou MD, PhD, Spyridon Skaltsas MD, PhD, Christos Kittas MD, PhD & Antonios Koutselinis MD, PhD

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  1. Stamatios E. Theocharis MD
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  2. Alexandra P. Margeli PhD
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  3. Chara Spiliopoulou MD, PhD
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  4. Spyridon Skaltsas MD, PhD
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  5. Christos Kittas MD, PhD
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  6. Antonios Koutselinis MD, PhD
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Theocharis, S.E., Margeli, A.P., Spiliopoulou, C. et al. Hepatic stimulator substance administration enhances regenerative capacity of hepatocytes in cadmium-pretreated partially hepatectomized rats. Digest Dis Sci 41, 1475–1480 (1996). https://doi.org/10.1007/BF02088575

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  • DOI: https://doi.org/10.1007/BF02088575

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