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Evaluation of cytotoxicity in cultured cells by enzyme leakage

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Journal of tissue culture methods

Summary

The measurement of cell injury or cytotoxicity produced by chemicals or toxicants in cell culture has routinely been evaluated by such criteria as plating efficiency, cell growth, protein or DNA synthesis, and cell viability. We describe a more sensitive and earlier index of cytotoxicity: leakage of cytoplasmic enzymes from injured cells into the culture medium.

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Authors and Affiliations

  1. Department of Pharmacology College of Pharmacy, The University of Texas, 78712, Austin, Texas, USA

    David B. Mitchell, Kenneth S. Santone & Daniel Acosta

Authors
  1. David B. Mitchell
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  2. Kenneth S. Santone
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  3. Daniel Acosta
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Mitchell, D.B., Santone, K.S. & Acosta, D. Evaluation of cytotoxicity in cultured cells by enzyme leakage. Journal of Tissue Culture Methods 6, 113–116 (1980). https://doi.org/10.1007/BF02082861

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  • DOI: https://doi.org/10.1007/BF02082861

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