Abstract
Intracarotid injection of B16-B14b or B16-B15b melanoma cells, previously established from B16-F1 melanoma byin vivo selection fourteen- or fifteentimes, respectively, for brain surface colonization, preferentially produced tumor nodules in mice at brain surface sites, most frequently in the dura mater, followed by the leptomeninges and cerebral cortex. There was a marked difference, however, in tumor growth at these sites using the two B16 sublines. Intracarotid injection of B16-B14b cells rarely produced visible tumors, whereas B16-B15b cells formed deeply pigmented tumors up to 7 mm in diameter in the brain meninges of almost all mice examined. Histologic and electron microscopic investigation revealed that B16-B14b tumors evoked dramatic immunocyte cell infiltration and granulomatous reactions, while B16-B15b tumors were accompanied by much less tumor-host cell reactions. Splenectomy or laparotomy 1–2 weeks before or after intracarotid injection of B16-B14b cells dramatically enhanced tumor growth in the dura mater without extensive tumor-host cell reactions. The results suggest that the differential growth of B16-B14b and B16-B15b tumor cells in the cerebral dura mater is based, in part, on the abilities of these melanoma cells to elicit host cell reactions.
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Brunson, K. W., Beattie, G., andNicolson, G. L., 1978, Selection and altered tumor cell properties of brain-colonizing metastatic melanoma.Nature (London),272, 543–545.
Ferrer, J. F., 1968, Enhancement of the growth of sarcoma 180 in splenectomized and sham-operated AKR mice.Transplantation,6, 160–166.
Fidler, I. J., 1973, Selection of successive tumor lines for metastasis.Nature (London),242, 148–149.
Fisher, B., andFisher, E. R., 1959, Experimental studies of factors influencing hepatic metastases III. Effect of surgical trauma with special reference to liver injury.Annals of Surgery,150, 731–744.
Grain, G. O., andKarr, J. P., 1955, Diffuse leptomeningeal carcinomatosis. Clinical and pathological characteristics.Neurology,5, 706–722.
Grooms, G. A., Eilber, F. R., andMorton, D. L., 1977, Failure of adjuvant immunotherapy to prevent central nervous system metastasis in malignant melanoma patients.Journal of Surgical Oncology,9, 147–153.
Kawaguchi, T., Kawaguchi, M., Miner, K. M., Lembo, T. M., andNicolson, G. L., 1983, Brain meninges tumor formation byin vivo-selected metastatic B16 melanoma variants in mice.Clinical and Experimental Metastasis,1, 247–259.
Kawaguchi, T., Kawaguchi, M., Dulski, K., andNicolson, G. L., 1985, Cellular behavior of metastatic B16 melanoma and cerebral invasion: An electron microscopic study.Invasion and Metastasis,5, 16–30.
Kokkoris, C. P., 1983, Leptomeningeal carcinomatosis: how does cancer reach the pia-arachnoid?Cancer,51, 154–160.
Ludatsher, R. M., Luse, S. A., andSuntzeff, V., 1967, An electron microscopic study of pulmonary tumor emboli from transplantable Morris hepatoma 5123.Cancer Research,27, 1939–1952.
Milas, L., andMujagic, H., 1973, The effect of splenectomy on fibrosarcoma ‘metastasis’ in lungs of mice.International Journal of Cancer,11, 186–190.
Miner, K. M., Lotan, R., andNicolson, G. L., 1981, Metastatic and melanogenic properties ofin vivo-selected B16 melanoma sublines and their clonal derivatives.Phenotypic Expression in Pigmented Cells, edited by M. Seiji (Tokyo: University of Tokyo Press), pp. 529–532.
Miner, K. M., Kawaguchi, T., Uba, G. W., andNicolson, G. L., 1982, Clonal drift of cell surface, melanogenic, and experimental metastatic properties ofin vivo-selected, brain meninges-colonizing murine B16 melanoma.Cancer Research,42, 4631–4638.
Miner, K. M., Klostergaard, J., Granger, G. A., andNicolson, G. L., 1983, Differential cytolytic and cytostatic effect of inducible factor(s) released from the murine monocytic cell line J774 on metastatic variants of B16 melanomain vitro.Journal of the National Cancer Institute,70, 717–724.
Nakashima, Y., Kawaguchi, T., andNakamura, K., 1985, The mechanisms of metastasis formation in injured parietal peritoneum by Yoshida sarcoma cells: An electron microscopic study.Fukushima Journal of Medical Science,31, 17–28.
Nicolson, G. L., 1982, Cancer metastasis: organ colonization and the cell surface properties of malignant cells.Biochimica et Biophysica Acta,695, 113–176.
Nicolson, G. L., 1987, Tumor cell instability, diversification and progression to the metastatic phenotype: from oncogene to oncofetal expression.Cancer Research,47, 1473–1487.
Nicolson, G. L., Brunson, K. W., andFidler, I. J., 1978, Specificity of arrest, survival and growth of selected metastatic variant cell lines.Cancer Research,38, 4105–4111.
Nicolson, G. L., andDulski, K. M., 1986, Organ specificity of metastatic tumor colonization is related to organ-selective growth properties of malignant cells.International Journal of Cancer,38, 289–294.
Nicolson, G. L., Kawaguchi, T., Kawaguchi, M., andVan Pelt, C., 1986, Brain surface invasion and metastasis of murine malignant melanoma.Journal of Neuro-Oncology,4, 209–218.
Posner, J. B., 1980, Brain metastasis: A clinician's view.Brain Metastasis, edited by L. Weiss, H. A. Gilber and J. B. Posner (Boston: G. K. Hall), pp. 2–29.
Raz, A., andHart, I. R., 1980, Murine melanoma: a model for intracranial metastasis.British Journal of Cancer,42, 331–341.
Ron, Y., Gorelik, E., Feldman, M., andSegal, S., 1982, Effect of splenectomy on the progression of postoperative pulmonary metastases of the 3LL tumor.European Journal of Cancer and Clinical Oncology,18, 391–397.
Roos, E., andDingemans, K. P., 1979, Mechanisms of metastasis.Biochimica et Biophysica Acta,560, 135–166.
Sato, H., andSuzuki, M., 1977, Metastasis and chemotherapy, with reference to permeability of the microcirculation system.Cancer Invasion and Metastasis: Biologic Mechanisms and Therapy, edited by S. P. Day, W. P. L. Myers, S. Sansly, S. Garattini and M. G. Lewis (New York: Raven Press), pp. 145–149.
Tobai, S., Kawaguchi, T., Asahina, S., andNakamura, K., 1980, Some findings on the intravasation of Yoshida sarcoma cells in the omentum.Gann,71, 578–579.
Ushio, Y., Chernik, N. L., Shapiro, W. R., andPosner, J. B., 1977, Metastatic tumor of the brain: development of an experimental model.Annals of Neurology,2, 20–29.
Weiss, L., 1983, Random and non-random processes in metastasis, and metastatic inefficiency.Invasion and Metastasis,3, 193–207.
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Kawaguchi, T., Kawaguchi, M., Lembo, T.M. et al. Differential tumor growth of blood-borne B16 melanoma variants in cerebral dura mater is related to tumor-host cell reactions. Clin Exp Metast 7, 1–14 (1989). https://doi.org/10.1007/BF02057177
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DOI: https://doi.org/10.1007/BF02057177