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Studies on Aspergilli XVI. Effect of pH, temperature, and carbon and nitrogen interaction

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Summary

The effect of pH, temperature, and carbon and nitrogen interaction on the growth and sporulation ofAspergillus nidulans (Eidam)Wint.,A. rugulosus Thom &Raper,A. variecolor (Berk. &Br.)Thom &Raper andA. quadrilineatus was studied. All the moulds could grow on a wide range of pH (2.0 to 12.0) but the growth was poor on too acid and too alkaline media. Best growth ofA. rugulosus, A. quadrilineatus, andA. violaceus was seen at pH 6.5 and that ofA. nidulans andA. variecolor at pH 7.0. In general maximum production of perithecia was recorded between pH 6.0 and 8.0.

All the above species ofAspergillus under study could grow between a temperature range of 10° C–48° C, but the growth was poor at 10° C and 48° C. The present moulds showed good growth at 20° C, 25°C, and 30° C. At 40° CA. nidulans andA. rugulosus showed moderate growth while the rest of the Aspergilli attained good growth. Temperatures between 20° C–30° C favoured excellent perithecial production.

In general, little improvement in growth was noted on media containing good carbon and nitrogen sources. Malic acid was found to be useless when supplied singly. But, poor growth was recorded when supplied in combination with amino acids, amide, and peptone. This was due to the fact that these N sources also supplied carbon for their metabolism.

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Author notes

  1. V. P. Agnihotri

    Present address: Department of Botany and Plant Pathology, Auburn University, Auburn, Alabama, USA

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  1. P. O. Rudrapur, Dist. Nainital, India

    V. P. Agnihotri

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  1. V. P. Agnihotri
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Agnihotri, V.P. Studies on Aspergilli XVI. Effect of pH, temperature, and carbon and nitrogen interaction. Mycopathologia et Mycologia Applicata 24, 305–314 (1964). https://doi.org/10.1007/BF02053641

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