Skip to main content
SpringerLink
Log in

Spironolactone-induced proliferation of smooth-surfaced endoplasmic reticulum in the liver of rats

  • Published:
Zeitschrift für die gesamte experimentelle Medizin einschließlich experimentelle Chirurgie

Summary

Electron microscopic studies revealed that spironolactone, given orally to rats, causes a marked proliferation of smooth-surfaced endoplasmic reticulum in hepatocytes. The characteristic fine structural alterations in the liver may represent the morphologic equivalent of stimulation or induction of drug metabolizing microsomal enzymes and may explain why spironolactone protects rats against the injurious effects of different compounds.

Zusammenfassung

Elektronenmikroskopische Untersuchungen zeigten bei der Ratte nach oral verabreichtem Spironolacton in den Hepatocyten eine starke Wucherung der glatten Oberflächen des endoplasmischen Reticulum. Die charakteristischen, feinen Strukturveränderungen in der Leber stellen möglicherweise das morphologische äquivalent einer Stimulierung oder Bildung von Enzymen dar, die körperfremde Stoffe metabolisieren. Damit ließe sich erklären, warum Spironolacton die Ratte gegen schädigende Einwirkungen verschiedener Substanzen zu schützen vermag.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arcasoy, M. M., Smuckler, E. A.: Acute effects of digoxin intoxication on rat hepatic and cardiac cells. Structural and functional changes in the endoplasmic reticulum. Lab. Invest.20, 190–201 (1969).

    PubMed  CAS  Google Scholar 

  • Azarnoff, D. L., Grady, H. J., Svoboda, D. J.: The effect of DDD on barbiturate and steroid-induced hypnosis in the dog and rat. Biochem. Pharmacol.15, 1985–1993 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Burger, P. C., Herdson, P. B.: Phenobarbital-induced fine structural changes in rat liver. Amer. J. Path.48, 793–809 (1966).

    PubMed  CAS  Google Scholar 

  • Conney, A. H.: Pharmacological implications of microsomal enzyme induction. Pharmacol. Rev.19, 317–366 (1967).

    PubMed  CAS  Google Scholar 

  • Fawcett, D. W.: Observations on the cytology and electron microscopy of hepatic cells. J. nat. Cancer Inst.15, 1475–1503 (1955).

    PubMed  CAS  Google Scholar 

  • Fouts, J. B., Rogers, L. A.: Morphological changes in the liver accompanying stimulation of microsomal drug metabolizing enzyme activity by phenobarbital, chlordane, benzpyrene or methyl-cholanthrene in rats. J. Pharmacol. exp. Ther.147, 112–119 (1965).

    PubMed  CAS  Google Scholar 

  • Kovács, K., Somogyi, A.: Prevention by spironolactone of 7,12-dimethylbenz(a)-anthracene-induced adrenal necrosis. Proc. Soc. exp. Biol. (N.Y.)131, 1350–1352 (1969).

    Google Scholar 

  • Kuntzman, R.: Drugs and enzyme induction. Ann. Rev. Pharmacol.9, 21–36 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Orrenius, S., Ericsson, J. L. E., Ernster, L.: Phenobarbital-induced synthesis of the drug-metabolizing enzyme system and its relationship to the proliferation of endoplasmic membranes. A morphological and biochemical study. J. Cell Biol.25, 627–639 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Ortega, P.: Light and electron microscopy of dichlorodiphenyltrichloroethane (DDT) poisoning in the rat liver. Lab. Invest.15, 657–679 (1966).

    PubMed  CAS  Google Scholar 

  • Porter, K. R., Bonneville, M. A.: Fine Structure of Cells and Tissues. Third edition, pp. 55–65. Philadelphia: Lea and Febiger 1968.

    Google Scholar 

  • Remmer, H., Merker, H. J.: Drug-induced changes in the liver endoplasmic reticulum; association with drug-metabolizing enzymes. Science142, 1657–1658 (1963a).

    Article  PubMed  CAS  Google Scholar 

  • — —: Enzyminduktion und Vermehrung von endoplasmatischem Reticulum in der Leberzelle während der Behandlung mit Phenobarbital (Luminal). Klin. Wschr.41, 276–283 (1963b).

    Article  PubMed  CAS  Google Scholar 

  • — —: Effect of drugs on the formation of smooth endoplasmic reticulum and drugmetabolizing enzymes. Ann. N.Y. Acad. Sci.123, 79–97 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Selye, H., Jelínek, J., Krajńy, M.: Prevention of digitoxin poisoning by various steroids. J. Pharm. Sci.58, 1055–1059 (1969a).

    Article  PubMed  CAS  Google Scholar 

  • —, Krajńy, M., Savoie, L.: Digitoxin poisoning: prevention by spironolactone. Science164, 842–843 (1969b).

    Article  PubMed  CAS  Google Scholar 

  • —, Mécs, I., Savoie, L.: Inhibition of anesthetics and sedative actions by spironolactone. Anesthesiology31, 261–264 (1969c).

    Article  PubMed  CAS  Google Scholar 

  • Solymoss, B., Classen, H. G., Varga, S.: Increased hepatic microsomal activity induced by spironolactone and other steroids. Proc. Soc. exp. Biol. (N.Y.)132, 940–942 (1969).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut de Médecine et de Chirurgie expérimentales, Université de Montréal, Montréal, Canada

    K. Kovács, J. A. Blascheck & C. Gardell

Authors
  1. K. Kovács
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. A. Blascheck
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Gardell
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kovács, K., Blascheck, J.A. & Gardell, C. Spironolactone-induced proliferation of smooth-surfaced endoplasmic reticulum in the liver of rats. Z. Gesamte Exp. Med. 152, 104–110 (1970). https://doi.org/10.1007/BF02046596

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02046596

Key-words

Schlüsselwörter

Search

Navigation