Abstract
Oral administration of two isomeric tetrachlorobiphenyls (TCBs) (2, 3, 4, 5-and 3, 5, 3′, 5′-tetrachlorobiphenyls) to rats at three dosage levels does not cause any adverse effect on their growth. However, several hepatic enzyme systems are affected, even at the lowest level (5 mg/kg). EthylmorphineN-demethylase and aniline hydroxylase activities are slightly induced by 2, 3, 4, 5-TCB but, at a medium dose level (20 mg/kg), the activity ofpara-nitroanisoleO-demethylase is induced two-fold. Also, 3, 5, 3′, 5′-TCB induces these enzyme systems in male rats but decreases the activities of aniline hydroxylase and ethyl morphineN-demethylase in female rats at higher doses. An extra polar metabolite occurs in thein vitro metabolism of oestradiol-3H when female rats are treated with 2, 3, 4, 5-TCB at a dosage level of 40 mg/kg. UDP-Glucuronyl/transferase and δ-aminolevulinic acid synthetase activities are not affected by the administration of these two TCBs.
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References
Conney, A. H., J. R. Gillette, J. K. Inscoe, E. G. Trams, and H. S. Posner: Induced synthesis of liver microsomal enzymes which metabolize foreign compounds. Science130, 1478 (1959).
Duke, T. W., J. I. Lowe and S. J. Wilson, Jr.: A polychlorinated biphenyl (Aroclor 1284) in the water, sediment, and biota of Escambia Bay, Florida. Bull. Environ. Contam. Toxicol.5, 171 (1970).
Hogeboom, G. H.: Fractionation of cell components of animal tissues. In Colowick, S.P. and N. O. Kaplan (eds.): Methods in enzymology, pp. 16. New York: Academic Press (1962).
Holmes, T. W., J. H. Simmons, and J. O. Tatton: Chlorinated hydrocarbons in British wildlife. Nature216, 227 (1967).
Jensen, S.: Report of a new chemical hazard. New Sci.32, 612 (1966).
Jensen, S., A. G. Johnels, M. Olsson and G. Otterlind: DDT and PCT in marine animals from Swedish waters. Nature224, 247 (1969).
Kato, R. and J. R. Gillette: Effects of starvation on NADPH-dependent enzymes in liver microsomes of male and female rats. J. Pharmacol. Exp. Ther.150, 279 (1965).
Kato, R. and M. Takayanaghi: Differences among the action of phenobarbital, methylcholanthrene and male sex hormones on microsomal drug-metabolizing enzyme systems of rat liver. Jap. J. Pharmacol.16, 380 (1966).
Keil, J. E., L. E. Priester, and S. H. Sandifer: Polychlorinated biphenyl (Aroclor 1242): Effects of uptake on growth, nucleic acids and chlorophyll of a marine diatom. Bull. Environ. Contam. Toxicol.6, 156 (1971).
Kuratsune, M., T. Yoshimura, J. Matsuzaka, and A. Yamaguchi: Epidemiologic study on Yusho, a poisoning caused by ingestion of rice oil contaminated with a commercial brand of polychlorinated biphenyls. Environmental Health Perspectives1, 119 (1972).
Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randal: Protein measurement with the folin phenol reagent. J. Biol. Chem.193, 265 (1951).
Lucier, G. W., O. S. McDaniel, and H. B. Matthews: Microsomal rat liver UDP-glucuronyl-transferase: Effect of piperonyl butoxide and other factors on enzyme activity. Arch. Biochem. Biophys.145, 520 (1971).
Mehendale, H. M., P. R. Chen, L. Fishbein, and H. B. Matthews: Unpublished data (1972a).
Mehendale, H. M., L. Fishbein, M. Fields, and H. B. Matthews: Fate of mirex-14C in the rat and uptake in plants, Bull. Environ. Contam. Toxicol.8, 200 (1972b).
Nowicki, H. G. and A. W. Norman: Enhanced hepatic metabolism of testosterone, 4-androstene-3, 17-dione, and estradiol-17β in chickens pretreated with DDT or PCB. Steroids19, 85 (1972).
Oswald, E. O.,et al. Unpublished data (1972).
Pardini, R. S.: Polychlorinated biphenyls (PCBs): Effect on mitochondrial enzyme systems. Bull. Environ. Contam. Toxicol.6, 539 (1971).
Risebrough, R. W., P. Rieche, D. B. Peakall, S. G. Herman, and M. N. Kirven: Polychlorinated biphenyls in the global ecosystem. Nature220, 1098 (1968).
Schenkman, J. B., H. Remmer, and R. W. Estabrook: Spectral studies of drug interaction with hepatic microsomal cytochrome. Mol. Pharmacol.3, 113 (1967).
Sell, J. L., K. L. Davison, and K. B. Poonacha: Decreased aniline hydroxylase activity in Japanese quail due to dietary DDT. J. Agr. Food Chem.20, 553 (1972).
Street, J. C., F. M. Urry, D. J. Wagstaff, and A. D. Blau: Comparative effects of polychlorinated biphenyl and organochlorine pesticides in induction of hepatic microsomal enzymes. Paper (Pest. 17) presented at the 158th American Chemical Society meeting, New York, N. Y. (1969).
Urata, G. and S. Granick: Biosynthesis of α-aminoketones and the metabolism of aminoketones, J. Biol. Chem.238, 811 (1963).
Venkatesan, N., J. C. Areos, and M. F. Argus: Differential effect of polycyclic hydrocarbons on the demethylation of the carcinogen dimethylnitrosamine by rat tissues. Life Sci.7(I), 1111 (1968).
Villeneuve, D. C., D. L. Grant, W. E. J. Philips, M. L. Clark, and D. J. Clegg: Effects of PCB administration on microsomal enzyme activity in pregnant rabbits. Bull. Environ. Contam. Toxicol.6, 120 (1971).
Vos, J. G. and R. B. Beems: Dermal toxicity studies of technical polychlorinated biphenyls and fractions thereof in rabbits. Toxicol. Appl. Pharmacol.19, 617 (1971).
Vos, J. G. and J. H. Koeman: Comparative toxicologic study with polychlorinated biphenyls in chicks with special reference to porphyria, edema formation, liver necrosis and tissue residues. Toxicol. Appl. Pharmacol.17, 656 (1970).
Vos, J. G. and E. Notenboom-Ram: Toxicology of PCBs for mammals and for birds. Environmental Health Perspectives1, 105 (1972).
Vos, J. G., J. J. T. W. A. Strik, C. W. M. Van Holsteyn, and J. H. Pennings: Polychlorinated biphenyls as inducers of hepatic porphyria in Japanese quail, with special reference to δ-aminolevulinic acid synthetase activity, fluorescence, and residues in the liver. Toxicol. Appl. Pharmacol.20, 232 (1971);
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Chen, P.R., Mehendale, H.M. & Fishbein, L. Effect of two isomeric tetrachlorobiphenyls on rats and their hepatic enzymes. Arch. Environ. Contam. Toxicol. 1, 36–47 (1973). https://doi.org/10.1007/BF02030144
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DOI: https://doi.org/10.1007/BF02030144