Skip to main content
SpringerLink
Log in

Inhibition of platelet phospholipase-A2 as a mechanism for the anti-aggregating effect of linoleic acid

  • Platelets and Thrombosis
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

Linoleic acid was incorporated into platelet phospholipids and then released after activation of phospholipase A2 (PL-A2) with thrombin or ionophore A23187. The rate of this release was tenfold lower for linoleic than for arachidonic acid. This observation strongly suggests that incorporation of linoleic acid in platelet phospholipids might inhibit platelet PL-A2 and might explain the anti-aggregating effect of linoleic acid. In fact it has also been shown that linoleic acid inhibits PL-A2 activity at concentrations which antagonize platelet aggregation. Moreover, in experimental conditions where platelet cyclo-oxygenase is totally inhibited, aggregation does occur and can still be blocked by linoleic acid. This latter observation led to the conclusion that the anti-aggregating effect of linoleic acid is independent from prostaglandin pathway and is probably related to the phospholipid metabolism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. E. Böhle, J. Bauke, E. Hartmuth andK. Breddin,Untersuchungen über di Agglutination der Blutplättchen nach Zufuhr vershiedener Nahrungsfette, Klin Wschr.43, 555 (1965).

    Article  PubMed  Google Scholar 

  2. S. Renaud, K. Kuba, C. Goulet, Y. Lemire andC. Allard,Relationship Between Fatty Acid Composition of Platelets and Platelet Aggregation in Rat and Man, Circ. Res.26, 553 (1970).

    PubMed  Google Scholar 

  3. G. Hornstra,The Influence of Dietary Sunflower Seed Oil and Hardened Coconut Oil on Intra-arterial Occlusive Thrombosis in Rats, Nutr. Metabol.13, 140 (1971).

    Google Scholar 

  4. J. Gerrard, J. White andW. Krivit,Labile Aggregation Stimulating Substance, Free Fatty Acids, and Platelet Aggregation, J. Lab. Clin. Med.87, 73 (1976).

    PubMed  Google Scholar 

  5. M. Silver, J. Smith, C. Ingerman andJ. Kocsis,Arachidonic Acid Induced Human Platelet Aggregation and Prostaglandin Formation, Prostaglandins4, 863 (1973).

    Article  PubMed  Google Scholar 

  6. G. Blackwell, W. Duncombe, R. Flower, M. Parson andJ. Vane,The Distribution and Metabolism of Arachidonic Acid in Rabbit Platelets During Aggregation and its Modification by Drugs, Br. J. Pharmac.59, 353 (1977).

    Google Scholar 

  7. C. Pace-Asciak andL. Wolfe,Inhibition of Prostaglandin Synthesis by Oleic, Linoleic and Linolenic Acids, Biochim. Biophys. Acta152, 784 (1968).

    PubMed  Google Scholar 

  8. A. Weiss,The Pharmacology of Platelet Inhibition, in:Progress in Haemostasis and Thrombosis (Ed. T.H. Spaet; Grune and Stratton, Inc., New York, 1972), p. 199.

    Google Scholar 

  9. G.V.R. Born,Aggregation of Blood Platelets by Adenosine Diphosphate and its Reversal, Nature194, 927 (1962).

    PubMed  Google Scholar 

  10. T. Bills, J. Smith andM. Silver,Metabolism of [14 C]Arachidonic Acid by Human Platelets, Biochim. Biophys. Acta424, 303 (1976).

    PubMed  Google Scholar 

  11. W. Pickett, R. Jesse andP. Cohen,Initiation of Phospholipase A 2 Activity in Human Platelets by the Calcium Ionophore A 23187, Biochim. Biophys. Acta486, 209 (1977).

    Google Scholar 

  12. S. Rittenhouse-Simmons andD. Deykin,The Mobilization of Arachidonic Acid in Platelets Exposed to Thrombin or Ionophore A 23187, J. Clin. Invest.60, 495 (1977).

    PubMed  Google Scholar 

  13. T. Bills, J. Smith andM. Silver,Selective Release of Arachidonic Acid from the Phospholipids of Human Platelets in Response to Thrombin, J. Clin. Invest.60, 1 (1977).

    PubMed  Google Scholar 

  14. G. Ansell andJ. Hawthorne, Eds., in:Phospholipids, vol. III (Elsevier Publishing Company, 1964), p. 34.

  15. H. Bult andI. Bonta,Rat Platelets Aggregate in Absence of Endogenous Precursor of Prostaglandin Endoperoxides, Nature264, 449 (1976).

    Article  PubMed  Google Scholar 

  16. B. Vargaftig,Carrageenan and Thrombin Trigger Prostaglandin Synthetase-Independent Aggregation of Rabbit Platelets: Inhibition by Phospholipase A 2 Inhibitors, J. Pharm. Pharmac.29, 222 (1977).

    Google Scholar 

  17. E. Lapetina, K. Chandrabose andP. Cuatrecasas,Ionophore A 23187 and Thrombin Induced Platelet Aggregation: Independence from Cycloxygenase Products, Proc. Natl. Acad. Sci.75, 818 (1978).

    PubMed  Google Scholar 

  18. M. Chignard, J.P. Le Couedic, M. Tence, J. Benveniste andB. Vargaftig,The Role of Platelet-Activating Factor in Platelet Aggregation, Nature279, 799 (1979).

    Article  PubMed  Google Scholar 

  19. J. Benveniste, J.P. Le Couedic, J. Polonsky andM. Tence,Structural Analysis of Purified Platelet-Activating Factor by Lipase, Nature269, 170 (1977).

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre de Recherches Clin-Midy, Rue du Professeur Joseph Blayac, 34082, Montpellier-Cédex, France

    E. Vallee, J. Gougat & M. Ageron

Authors
  1. E. Vallee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Gougat
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Ageron
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vallee, E., Gougat, J. & Ageron, M. Inhibition of platelet phospholipase-A2 as a mechanism for the anti-aggregating effect of linoleic acid. Agents and Actions 10, 57–62 (1980). https://doi.org/10.1007/BF02024179

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02024179

Keywords

Search

Navigation