Abstract
The performance of four miniaturized biochemical test systems (API 20C Auxanogram, Mycotube, Oricult, and Uni-Yeast-Tek) for the identification of yeasts was evaluated using standard biochemical tests as reference. One hundred and twenty-six strains were used for the study. Mycotube identified 93 % of the strains correctly, and Uni-Yeast-Tek 89 %. API 20C Auxanogram and Oricult identified 77 % and 82 % of the strains respectively if the germ tube test was also included. Mycotube and Uni-Yeast-Tek in particular facilitate identification of yeasts in the routine clinical laboratory but recourse to microscopic examination of morphology and other supplementary tests may also be necessary.
Similar content being viewed by others
References
Lodder, J. (ed.): The yeasts. North-Holland Publishing Co., Amsterdam, 1970.
De Louvois, J., Mulhall, A., Hurley, R.: Biochemical identification of clinically important yeasts. Journal of Clinical Pathology 1979, 32: 715–718.
Buesching, W. J., Kurek, K., Roberts, G. D.: Evaluation of the modified API 20C system for identification of clinically important yeasts. Journal of Clinical Microbiology 1979, 9: 565–569.
Dermoumi, H.: Die Bestimmung von hefeähnlichen Pilzen aus klinischem Untersuchungsmaterial mit dem API 20C Auxanogram. Das Ärztliche Laboratorium 1979, 25: 289–291.
Shinoda, T., Kaufman, L., Padhye, A. A.: Comparative evaluation of the iatron serological Candida check-kit and the API 20C kit for identification of medically importantCandida species. Journal of Clinical Microbiology 1981, 13: 513–518.
Huppert, M., Haper, G., Sun, S. H., Delanerolle, V.: Rapid methods for identification of yeasts. Journal of Clinical Microbiology 1975, 2: 21–34.
Dickgiesser, N., Pieringer, E.: Suitability of the modified API 20C, Mycotube and Bacto-Candida albicans-antiserum for the identification of yeasts in the routine laboratory. Zentralblatt der Bakteriologie und Hygiene (A) 1980, 247: 132–137.
Dermoumi, H.: Mycotube — ein neues Hilfsmittel zur Bestimmung medizinisch bedeutsamer Hefen. Das Ärztliche Laboratorium 1978, 24: 79–82.
Gille, Y., Guinet, R., Terraillon, J.: Evaluation d'une méthode simplifiée (Mycotube R) d'identification de levure. Médecine et Maladies Infectieuses 1981, 11: 269–274.
Miyamoto, C., Okuda, T., Ikeda, A., Marujama, H. B.: Functional evaluation of „Mycotube“ Roche, a new kit for taxonomical identification of pathogenic yeasts. Japanese Journal of Medical Mycology 1980, 21: 109–121.
Bowman, P. I., Ahearn, D. G.: Evaluation of the Uni-Yeast-Tek kit for the identification of medically important yeasts. Journal of Clinical Microbiology 1975, 2: 354–358.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bergan, T., Hollum, A.B. & Vangdal, M. Evaluation of four commercial biochemical test systems for identification of yeasts. Eur. J, Clin. Microbiol. 1, 217–222 (1982). https://doi.org/10.1007/BF02019711
Issue Date:
DOI: https://doi.org/10.1007/BF02019711