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Thymidine incorporation in saltern ponds of different salinities: Estimation of in situ growth rates of halophilic archaeobacteria and eubacteria

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Abstract

Incorporation of [methyl-3H]thymidine was measured in solar saltern ponds of different salinities. Estimated doubling times of the bacterial communities were in the range of 1.1 to 22.6 days. Even at the highest salt concentrations (NaCl saturation), relatively rapid thymidine incorporation was observed. In an attempt to differentiate between activity of halophilic archaeobacteria (theHalobacterium group) and halophilic eubacteria, taurocholate, which causes lysis of the halobacteria without affecting eubacteria, was used. At salt concentrations exceeding 250 g/liter all thymidine incorporation activity could be attributed to halobacteria. Aphidicolin, a potent inhibitor of DNA synthesis in halobacteria, completely abolished thymidine incorporation at the highest salinities, but also caused significant inhibition at salinities at which halobacteria are expected to be absent. Attempts to use nalidixic acid to selectively inhibit DNA synthesis by the eubacterial communities were unsuccessful.

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References

  1. Forterre P, Elie C, Kohiyama M (1984) Aphidicolin inhibits growth and DNA synthesis in halophilic archaebacteria. J Bacteriol 159:800–802

    PubMed  Google Scholar 

  2. Fuhrman J, Azam F (1982) Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters: Evaluation and field results. Mar Biol 66:109–120

    Article  Google Scholar 

  3. Javor BJ (1983) Planktonic standing crop and nutrients in a saltern ecosystem. Limnol Oceanogr 28:153–159

    Google Scholar 

  4. Jordans MJ, Likens GE (1980) Measurement of planktonic bacterial production in an oligotrophic lake. Limnol Oceanogr 25:719–732

    Google Scholar 

  5. Karl DM (1986) Determination of in situ microbial biomass, viability, metabolism, and growth. In: Poindexter JS, Leadbetter ER (eds) Bacteria in nature, vol 2. Methods and special applications. Plenum Press, New York, pp 85–176.

    Google Scholar 

  6. Kushner DJ (1985) The Halobacteriaceae. In: Woese CR, Wolfe RS (eds) The bacteria—a treatise on structure and function, vol VIII: Archaebacteria. Academic Press, Orlando, pp 171–214

    Google Scholar 

  7. LaRock PA, Lauer RD, Schwarz JR, Watanabe KK, Wiesenburg DA (1979) Microbial biomass and activity distribution in an anoxic, hypersaline basin. Appl Environ Microbiol 37:466–470

    Google Scholar 

  8. Moriarty DJW, Pollard PC (1981) DNA synthesis as a measure of bacterial productivity in seagrass sediments. Mar Ecol-Prog Ser 5:151–156

    Google Scholar 

  9. Oren A (1989) Estimation of the contribution of halobacteria to the bacterial biomass and activity in solar salterns by the use of bile salts. FEMS Microbiol Ecol (in press)

  10. Oren A (1989) A method for the differential microscopic enumeration ofHalobacterium cells. J Microbiol Methods (in press)

  11. Oren A, Shilo M (1982) Population dynamics ofDunaliella parva in the Dead Sea. Limnol Oceanogr 27:201–211

    Google Scholar 

  12. Pedrini AM (1979) Nalidixic acid. In: Hahn FE (ed) Antibiotics, vol V/part 1. Mechanism of action of antibacterial agents. Springer-Verlag, Berlin, pp 154–175

    Google Scholar 

  13. Riemann B, Furhman J, Azam F (1982) Bacterial secondary production in freshwater measured by3H-thymidine incorporation method. Microb Ecol 8:101–114

    Article  Google Scholar 

  14. Riemann B, Søndergaard M (1984) Measurements of diel rates of bacterial secondary production in aquatic environments. Appl Environ Microbiol 47:632–638

    Google Scholar 

  15. Robarts RD, Wicks RJ (1989) [Methyl-3H]thymidine macromolecular incorporation and lipid labeling: Their significance to DNA labeling during measurements of aquatic bacterial growth rate. Limnol Oceanogr 34:213–222

    Google Scholar 

  16. Rodriguez-Valera F (1988) Characteristics and microbial ecology of hypersaline environments. In: Rodriguez-Valera F (ed) Halophilic bacteria, vol I. CRC Press, Boca Raton, pp 3–30

    Google Scholar 

  17. Rodriguez-Valera F, Ruiz-Berraquero F, Ramos-Cormenzana A (1981) Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentration. Microb Ecol 7:235–243

    Google Scholar 

  18. Schinzel R, Burger KJ (1984) Sensitivity of halobacteria to aphidicolin, an inhibitor of eukaryoticα-type DNA polymerases. FEMS Microbiol Lett 25:187–190

    Google Scholar 

  19. Wicks RJ, Robarts RD (1987) The extraction and purification of DNA labeled with [methyl-3H]thymidine in aquatic bacterial production studies. J Plankton Res 9:1159–1166

    Google Scholar 

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  1. The Division of Microbial and Molecular Ecology, The Institute of Life Sciences, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel

    Aharon Oren

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  1. Aharon Oren
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Oren, A. Thymidine incorporation in saltern ponds of different salinities: Estimation of in situ growth rates of halophilic archaeobacteria and eubacteria. Microb Ecol 19, 43–51 (1990). https://doi.org/10.1007/BF02015052

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