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An anti-human immunoglobulin M monoclonal antibody for detection of antibodies toToxoplasma gondii

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Abstract

An anti-human μ-chain monoclonal antibody, Tibi 82, was produced and tested for specificity by radioimmunoassay. Its reliability in detecting IgM antibodies toToxoplasma gondii was tested by two reverse immunosorbent methods (IgM-ISAGA and IgM-SPIHA) and the IgM fluorescent antibody test (IgM-IFA) on 400 sera. Whereas the results obtained with Tibi 82 and with two polyclonal reagents were highly correlated, the third commercial polyclonal reagent provided many false negative results. By standardizing IgM binding, Tibi 82 allowed the comparison of IgM-ISAGA with IgM-SPIHA on 100 sera: 17 % of the sera tested showed discrepancies due to the different toxoplasma antigens used. Although Tibi 82 facilitated the reading of results and enhanced sensitivity and specificity of the double-sandwich IgM-IFA method, the latter was still less sensitive than IgM-ISAGA with Tibi 82. Tests with the monoclonal antibody were consistently superior to tests with polyclonal antibodies.

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References

  1. Naot, Y., Remington, J. S.: An enzyme-linked immunosorbent assay for detection of IgM antibodies toToxoplasma gondii: use for diagnosis of acute acquired toxoplasmosis. Journal of Infectious Diseases 1980, 142: 757–766.

    PubMed  Google Scholar 

  2. Naot, Y., Desmonts, G., Remington, J. S.: IgM enzyme-linked immunosorbent assay test for the diagnosis of congenital toxoplasma infection. Journal of Pediatrics 1981, 98: 32–36.

    PubMed  Google Scholar 

  3. Naot, Y., Barnett, E. V., Remington, J. S.: Method for avoiding false-positive results occurring in IgM enzyme-linked immunosorbent assays due to presence of both rheumatoid factor and antinuclear antibodies. Journal of Clinical Microbiology 1981, 14: 73–78.

    PubMed  Google Scholar 

  4. Carosi, G., Filice, G., Meroni, V., Beloni, C., Gerola, O., Pesando, P.: Congenital toxoplasmosis: screening of 963 mothers and their children at birth. International Journal of Biological Research in Pregnancy 1981, 2: 117–122.

    PubMed  Google Scholar 

  5. Duermeyer, W., Van der Veen, J.: Specific detection of IgM-antibodies by ELISA, applied in hepatitis-A. Lancet 1978, ii: 684.

    Article  Google Scholar 

  6. Franco, E. L., Walls, K. W., Sulzer, A. J.: Reverse enzyme immunoassay for detection of specific anti-toxoplasma IgM antibodies. Journal of Clinical Microbiology 1981, 13: 859–864.

    PubMed  Google Scholar 

  7. Desmonts, G., Naot, Y., Remington, J. S.: Immunoglobulin M-immunosorbent agglutination assay for diagnosis of infectious diseases: diagnosis of acute congenital and acquired toxoplasma infections. Journal of Clinical Microbiology 1981, 14: 486–491.

    PubMed  Google Scholar 

  8. Desmonts, G.: IgM immunosorbent agglutination assay (ISAGA). In: Colloque international sur l'immunologie de la toxoplasmose. Lyon Médical, Lyon, 1982, p. 37–41.

    Google Scholar 

  9. Hermentin, K., Picher, O., Aspöck, H., Auer, H., Hassl, A.: A solid phase indirect haemadsorption assay (SPIHA) for detection of immunoglobulin M antibodies toToxoplasma gondii: application to diagnosis of acute acquired toxoplasmosis. Zentralblatt für Bakteriologie Mikrobiologie und Hygiene (A) 1983, 255: 380–391.

    Google Scholar 

  10. Forghani, B., Myoraku, C. K., Schmidt, N. J.: Production of monoclonal antibodies to human IgM for assay of viral IgM antibodies. Journal of Virological Methods 1982, 5: 317–327.

    Article  PubMed  Google Scholar 

  11. Remington, J. S., Desmonts, G.: Congenital toxoplasmosis: variability in the IgM fluorescent diagnosis. Journal of Pediatrics 1973, 83: 23–30.

    Google Scholar 

  12. Köhler, G., Milstein, C.: Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975, 256: 495–497.

    Article  PubMed  Google Scholar 

  13. Oi, V. T., Herzenberg, L. A.: Immunoglobulin producing hybrid cell lines. In: Mishell, B. B., Shiigi, S. M. (ed.): Selected methods in cellular immunology. W. H. Freeman and Company, San Francisco, 1980, p. 351–371.

    Google Scholar 

  14. Tsu, T. T., Herzenberg, L. A.: Solid-phase radioimmune assays. In: Mishell, B. B., Shiigi, S. M. (ed.): Selected methods in cellular immunology. W. H. Freeman and Company, San Francisco, 1980, p. 373–377.

    Google Scholar 

  15. Pouletty, Ph., Kadouche, J., Garcia-Gonzalez, M., Mihaesco, C., Najean, Y.: Use of monoclonal antibodies for precipitin reactions. In: Peeters, H. (ed.): Protides of the biological fluids. Volume 31. Pergamon Press, Oxford, 1984, p. 1063–1066.

    Google Scholar 

  16. Pinon, J. M., Gruson, N.: Interest of E.L.I.F.A. specific and compared immunological profiles in the early diagnosis of congenital toxoplasmosis. In: Colloque international sur l'immunologie de la toxoplasmose. Lyon Médical, Lyon, 1982, p. 27–30.

    Google Scholar 

  17. Sharma, S. D., Mullenax, J., Araujo, F. G., Erlich, H. A., Remington, J. S.: Western blot analysis of the antigens ofToxoplasma gondii recognized by human IgM and IgG antibodies. Journal of Immunology 1983, 131: 977–983.

    Google Scholar 

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Authors and Affiliations

  1. Service de Médecine Nucléaire, Hôpital Saint Louis, 2 Place du Dr. Fournier, 75010, Paris, France

    P. Pouletty & M. Garcia-Gonzalez

  2. Laboratoire de Parasitologie Mycologie, Hôpital de Maison Blanche, 51092, Reims, France

    J. M. Pinon & H. Thoannes

  3. Laboratoire de Sérologie Néonatale et de Recherche sur la Toxoplasmose, Institut de Puériculture de Paris, 26 Boulevard Brune, 75014, Paris, France

    G. Desmonts & P. Thulliez

  4. Unité de Recherche en Immunologie, Clonatec, 60, Rue de Wattignies, 75012, Paris, France

    J. Kadouche

Authors
  1. P. Pouletty
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  2. J. M. Pinon
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  3. M. Garcia-Gonzalez
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  4. G. Desmonts
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  5. P. Thulliez
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  6. H. Thoannes
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  7. J. Kadouche
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Pouletty, P., Pinon, J.M., Garcia-Gonzalez, M. et al. An anti-human immunoglobulin M monoclonal antibody for detection of antibodies toToxoplasma gondii . Eur. J, Clin. Microbiol. 3, 510–515 (1984). https://doi.org/10.1007/BF02013609

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  • DOI: https://doi.org/10.1007/BF02013609

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