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Ability of newer beta-lactam antibiotics to induce beta-lactamase production inEnterobacter cloacae

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  • Current Topic Inducible Beta-Lactamases: Enzymes of Increasing Clinical Importance
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Abstract

The beta-lactamase inducing properties of various new beta-lactam antibiotics in two isogenic strains ofEnterobacter cloacae were investigated. Beta-lactamase activity was measured two hours after addition of inducer to cells in the late logarithmic growth-phase. Beta-lactamase expression was highly dependent on the growth medium used, highest levels being obtained after induction with cefoxitin in Tryptic Soy broth, Mueller-Hinton broth and Nutrient broth. Upon induction the mutant 908 Ssi produced tenfold higher beta-lactamase levels than its parent wild type 908 Swi. Among the new antibiotics investigated, sulfoxides of several oxyimino-cephalosporins, HR 810, cefetamet, cefteram, carumonam and BRL 36650 were moderate or poor inducers. The penem FCE 22101 resembled imipenem in its strong inducing properties.

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  1. Pharmaceutical Research Division, F. Hoffmann-La Roche & Co. Ltd., Basel, Switzerland

    R. L. Then

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Then, R.L. Ability of newer beta-lactam antibiotics to induce beta-lactamase production inEnterobacter cloacae . Eur. J, Clin. Microbiol. 6, 451–455 (1987). https://doi.org/10.1007/BF02013109

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  • DOI: https://doi.org/10.1007/BF02013109

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