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A micromethod for the determination of fluoride in blood plasma and saliva

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Summary

A simple and accurate technique for the determination of fluoride (F−) in capillary-sampled blood is presented. The method is based on the known addition-slope determination technique using the fluoride electrode is required. A standard deviation of 1.3–5.6% in the range 300–10 ng F/ml was given by 259 duplicate determinations on human plasma. Measurements of parotid saliva showed that it was possible to analyze fluoride concentrations as low as 4 ng F/ml with good reproducibility. The results also show that a 150 μl sample is sufficient for fluoride determination with high accuracy. This method can easily be used in a clinical laboratory.

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Authors and Affiliations

  1. Department of Clinical Pharmacology, Karolinska Hospital, Stockholm, Sweden

    Jan Ekstrand

  2. Department of Cariology, Karolinska Institute, S-104 01, Stockholm, Sweden

    Jan Ekstrand

  3. Department of Pharmacology, Karolinska Institute, S-104 01, Stockholm, Sweden

    Jan Ekstrand

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  1. Jan Ekstrand
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Ekstrand, J. A micromethod for the determination of fluoride in blood plasma and saliva. Calc. Tis Res. 23, 225–228 (1977). https://doi.org/10.1007/BF02012789

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  • DOI: https://doi.org/10.1007/BF02012789

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