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Radiogenic free radicals as molecular probes in bone

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Summary

Exposure of bone mineral to X-rays generates free radicals. These are usually very labile, but can be stabilized at liquid nitrogen temperatures for study by electron spin resonance spectroscopy. The free radicals thus detected in the present study included one with resonances arising from an electron excess center and 2 species with electron-deficit centers: a phosphate anion radical and a radical associated with carbonate. Each of these radicals seemed to be located chiefly at the mineral surface and was sensitive to the surface environment. Presence of an organic phase, as in whole bone, modified free radical production in a manner that suggests interference with the formation of electron deficit centers. Comparison with other synthetic minerals suggests that precipitated carbonateapatites are good models for bone mineral.

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References

  1. Atkins, P.W., Symons, M.C.R.: The structure of inorganic radicals. Amsterdam: Elsevier 1967

    Google Scholar 

  2. Blumenthal, N.C., Betts, F., Posner, A.S.: Effect of carbonate and biological macromolecules on formation and properties of hydroxyapatite. Calcif. Tiss. Res.18, 81–90 (1975)

    Google Scholar 

  3. Brunauer, S., Emmett, P.H., Teller, E.: Adsorption of gases in multimolecular layers, J. Amer. Chem. Soc.60, 309–319 (1938)

    Google Scholar 

  4. Cevc, P., Schara, M., Ravnik, C.: Electron paramagnetic resonance study of irradiated tooth enamel. Radiat. Res.51, 581–589 (1973)

    Google Scholar 

  5. Chantry, G.W., Horsfield, A., Morton, J.R., Whiffen, D.H.: The structure, electron resonance and optical spectra of trapped CO3 − and NO3 −. Mol. Phys.5, 589–599 (1962)

    Google Scholar 

  6. Eachus, R.S., Symons, M.C.R.: Unstable intermediates. Part 1. The NO 3−3 impurity centre in irradiated calcium carbonate. J. chem. Soc. (A), 790–793 (1968)

  7. Fisher, B.V., Morgan, R.E., Phillips, G.O., Wardale, H.W.: Radiation damage in calcium phosphates and collagen: An interpretation of ESR spectra. Radiat. Res.46, 229–235 (1971)

    PubMed  Google Scholar 

  8. Gordy, W., Ard, W.B., Shields H.: Microwave spectroscopy of biological substances. I. Paramagnetic resonance in x-irradiated amino acids and proteins. Proc. Natl. Acad. Sci. U.S.,41, 983–996 (1955)

    Google Scholar 

  9. Hirschman, A., Sobel, A.E.: Composition of the mineral deposited during in vitro calcification in relation to the fluid. Arch. Biochem.110, 237–245 (1965)

    PubMed  Google Scholar 

  10. LeGeros, R.Z., Trautz, O.R., Klein, E., LeGeros, J.P.: Two types of carbonate substitution in the apatite structure. Experientia25, 5–7 (1969)

    PubMed  Google Scholar 

  11. Ostrowski, K., Dziedzic-Goclawska, A., Stachowicz, W., Michalik, J., Tarsoly, E., Komender, A.: Application of the electron spin resonance technique for quantitative evaluation of the resorption rate of irradiated bone grafts. Calcif. Tiss. Res.7, 58–66 (1971)

    Google Scholar 

  12. Ostrowski, K., Dziedzic/Goclawska, A., Stachowicz, W., Michalik, J.: Sensivity of the electron spin resonance technique as applied in histochemical research on normal and pathological calcified tissues. Histochemie32, 343–351 (1972)

    PubMed  Google Scholar 

  13. Ostrowski, K., Dziedzic-Goclawska, A., Sliwowski, A., Wojtajak, L., Michalik, K., Stachowicz, W.: Analysis of the crystallinity of calcium phosphate deposits in rat liver mitochondria by electron spin resonance spectroscopy. FEBS Letters60, 410–413 (1975)

    PubMed  Google Scholar 

  14. Pechauskas, R.A., Pullman, I.: Radiogenic free radicals in apatite: Influence of fluoride and hydroxide. Calcif. Tiss. Res.21, 121–128 (1976)

    Google Scholar 

  15. Serway, R.A., Marshall, S.A.: Electron spin resonance absorption spectra of CO3 − and CO 3−3 molecule-ions in irradiated single-crystal calcite. J. chem. Phys.46, 1949–1952 (1967)

    Google Scholar 

  16. Subramanian, S., Symons, M.C.R., Wardale, H.W.: Oxides and oxyions of the non-metals. Part XIII. Electron spin resonance studies of the PO4 − radical and related species in x-irradiated phosphates. J. chem. Soc. (A) 1239–1242 (1970)

  17. Termine, J.D., Eanes, E.D., Greenfield, D.J., Nylen M.U., Harper, R.A.: Hydrazine deproteinated bone mineral. Calcif. Tiss. Res.12, 73–90 (1973)

    Google Scholar 

  18. Termine, J.D., Posner, A.S.: Calcium phosphate formationin vitro. I. Factors affecting initial phase separation. Arch. Biochem. Biophys.140, 307–317 (1970)

    PubMed  Google Scholar 

  19. Treinin, A.: Trapped radicals in organic glasses. In: Radical ions (Kaiser, E., Kevan, L., eds.), p. 542–543. New York: Wiley-Interscience 1968

    Google Scholar 

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Authors and Affiliations

  1. Department of Radiology, New York Medical College, Fifth Avenue at 106th Street, 10029, New York, N.Y., USA

    R. A. Peckauskas & I. Pullman

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  1. R. A. Peckauskas
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  2. I. Pullman
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Peckauskas, R.A., Pullman, I. Radiogenic free radicals as molecular probes in bone. Calc. Tis Res. 25, 37–43 (1978). https://doi.org/10.1007/BF02010749

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  • DOI: https://doi.org/10.1007/BF02010749

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