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Bacterial ecology of an old-growth douglas fir canopy

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Abstract

Microbial populations associated with the major substrates of the canopy of a single 70 m old-growth Douglas fir were studied to determine potential activities. Seasonal samples from bark, foliage, epiphytic moss, lichens, and litter accumulations were collected to: (a) obtain population data, (b) isolate the major groups of microorganisms present, (c) measure enzymatic activities associated with cellulose and xylan degradation, and (d) examine the potential for nitrogen fixation. We tested 562 bacterial isolates for utilization of 25 compounds associated with the canopy substrates, and for activities in nitrogen and sulfur cycle transformations. Total bacterial populations, reflecting seasonal temperature and moisture conditions, were lowest on bark and foliage [21–266×103 colony-forming units (CFU/g)] and highest on moss and lodged litter (19–610×105 CFU/g). Lichens contained intermediate numbers of bacteria (3.3–270×105 CFU/g). The majority of the bacteria were classified as species ofArthrobacter, Bacillus, Flavobacterium, andXanthomonas. Isolates ofAlcaligenes (Achromobacter), Aeromonas, Chromobacterium, Micrococcus, andPseudomonas were less common. No measurable rates of nitrogen fixation attributable to free-living bacteria were detected by acetylene reduction. Eleven species in six genera of lichens containing a blue-green algal phycobiont showed positive acetylene reduction. One species,Lobaria oregana, accounted for 51% of the total lichen biomass of the canopy. Cellulase and xylanase activity was routinely detected in moss and litter samples, and less frequently in lichens. There was a strong correlation between the two activities for moss (r=0.94) and litter (r=0.81).

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References

  1. Aho, P. E., R. J. Seidler, H. J. Evans, and P. N. Raju: Distribution, enumeration, and identification of nitrogen-fixing bacteria associated with decay in living white fir trees. Phytopathology64, 1413–1420 (1974)

    Google Scholar 

  2. Asahina, Y., and S. Shibata: Chemistry of lichen substances. Japanese Society for the Promotion of Science, Ueno, Tokyo, Japan (1954)

    Google Scholar 

  3. Bernfield, P.: Amylasesα andβ Meth. Enzymol.1, 26–37 (1955)

    Google Scholar 

  4. Bernstein, M. E., and G. C. Carroll: Microbial populations on Douglas fir needle surface. Microb. Ecol.4, 41–52 (1977)

    Article  Google Scholar 

  5. Buchanan, R. E., and N. E. Gibbons (eds.): Bergey's Manual of Determinative Bacteriology, 8th Ed. Williams & Williams Co., Baltimore (1974)

    Google Scholar 

  6. Campbell, N. E. R., and H. J. Evans: Use of Pankhurst tubes to assay acetylene reduction by facultative and anaerobic nitrogen-fixing bacteria. Can. J. Microbiol.15, 1342–1343 (1969)

    PubMed  Google Scholar 

  7. Cooper, G., and G. C. Carroll: Ribitol as the major component of water-soluble leachates fromLobaria oregana. Bryologist (in press)

  8. Denison, R. F., B. Caldwell, B. Borman, L. Eldred, C. Swanberg, and S. Anderson: Effects of acid rain on nitrogen-fixation in Washington coniferous forests. In: Proceedings of the First International Symposium on Acid Precipitation and the Forest Ecosystem, edited by L. S. Dochinger and T. A. Seliga, pp. 993–949. U.S. For. Serv. Gen. Tech. Rep. NE-23, Washington, D.C. (1976)

  9. Denison, W. C.: Life in tall trees. Sci. Am.228(6), 74–80 (1973)

    PubMed  Google Scholar 

  10. Denison, W. C., D. M. Tracy, F. M. Rhoades, and M. Sherwood: Direct, non-destructive measurement of biomass and structure in living, old-growth Douglas fir. In: Research on Coniferous Forest Ecosystems, edited by J. F. Franklin, L. J. Dempster, and R. H. Waring, pp. 147–158. Proc. Symp. Northwest Sci. Assoc. (1972)

  11. Dobereiner, J., and J. M. Day: Associative symbioses in tropical grasses: characterization of microorganisms and dinitrogen fixation sites. Proc. Int. Symp. on N2 Fixation, pp. 518–538. Washington State University, Pullman (1974)

    Google Scholar 

  12. Dyrness, C. T., J. F. Franklin, and W. H. Moir: A preliminary classification of forest communities in the central portion of the western Cascades in Oregon. US/IBP Bulletin No. 4 Coniferous Forest Biome, University of Washington, Seattle (1974)

    Google Scholar 

  13. Gessel, S. P., R. B. Walker, and P. G. Haddock: Preliminary report on mineral deficiencies in Douglas fir. Soil Sci. Soc. Am. Proc.15, 364–369 (1951)

    Google Scholar 

  14. Goodfellow, M.: Properties and composition of the bacterial flora of a pine forest soil. J. Soil Sci.19, 154–167 (1968)

    Google Scholar 

  15. Grier, C. C., and R. S. Logan: Old-growthPseudotsuga menziesii communities of a western Oregon watershed: biomass distribution and production budgets. Ecol. Monogr.47, 373–400 (1977)

    Google Scholar 

  16. Hagedorn, C.: Influences of soil acidity onStreptomyces populations inhabiting forest soils. Appl. Environ. Microbiol.32, 368–375 (1976)

    PubMed  Google Scholar 

  17. Hardy, R. W. F., R. C. Burns, and R. D. Holsten: Applications of the acetylene-ethylene assay for measurement of nitrogen fixation. Soil Biol. Biochem.5, 47–81 (1973)

    Article  Google Scholar 

  18. Hissett, R., and T. R. G. Gray: Bacterial populations of litter and soil in a deciduous woodland: 1. Qualitative studies. Rev. Ecol. Biol. Sol.10, 495–508 (1973)

    Google Scholar 

  19. Holm, E., and V. Jensen: Aerobic chemoorganotrophic bacteria of a Danish beech forest. Oikos23, 248–260 (1972)

    Google Scholar 

  20. Jones, K.: Nitrogen fixation in the phyllosphere of the Douglas fir,Pseudotsuga douglasii. Ann. Bot.34, 239–244 (1970)

    Google Scholar 

  21. Jones, K.: Nitrogen-fixing bacteria in the canopy of conifers in a temperate forest. In: Microbiology of Aerial Plant Surfaces, edited by C. H. Dickinson and T. F. Preece, pp. 451–463. Academic Press Ltd., London (1976)

    Google Scholar 

  22. Jones, K., E. King, and M. Eastlick: Nitrogen fixation by free-living bacteria in the soil and in the canopy of Douglas fir. Ann. Bot.38, 765–772 (1974)

    Google Scholar 

  23. Jurgensen, M. F., and C. B. Davey: Non-symbiotic nitrogen-fixing microorganisms in forest and tundra soils. Plant Soil34, 341–356 (1971)

    Google Scholar 

  24. Kuster, E., and D. Gottlieb: The utilization of carbon compounds by someActinomycetales as an aid for species determination. J. Bacteriol.56, 107–114 (1948)

    Google Scholar 

  25. Kuster, E., and S. T. Williams: Selection of media for isolation of streptomycetes. Nature202, 928–929 (1964)

    PubMed  Google Scholar 

  26. Lenette, E. H., E. H. Spaulding, and J. P. Truant (eds.): Manual of Clinical Microbiology, 2nd Ed. American Society for Microbiology, Washington, D.C. (1974)

    Google Scholar 

  27. Millbank, J. W.: Lower plant associations. In: A Treatise on Dinitrogen Fixation, Section III: Biology, edited by R. W. F. Hardy and W. S. Silver, pp. 125–151. John Wiley & Sons, New York (1977)

    Google Scholar 

  28. Neilson, A. H., and L. Sparell: Acetylene reduction (nitrogen fixation) byEnterobacteriaceae isolated from paper mill process wastes. Appl. Environ. Microbiol.32, 197–205 (1976)

    Google Scholar 

  29. Niemela, S., and V. Sundman: Effects of clear-cutting on the composition of bacterial populations of northern spruce forest soils. Can. J. Microbiol.23, 131–138 (1977)

    PubMed  Google Scholar 

  30. Phillips, M. J.: Comparative rates of CO2 production from the forest floor in the Douglas fir ecosystem. M.S. thesis, Oregon State University, Corvallis (1975)

    Google Scholar 

  31. Pike, L. H., W. C. Denison, D. Tracy, M. Sherwood, and F. Rhoades: Floristic survey of epiphytic lichens and bryophytes growing on living, old-growth conifers in western Oregon. Bryologist78, 391–404 (1975)

    Google Scholar 

  32. Pike, L. H., R. A. Rydell, and W. C. Denison: A 400-year-old Douglas fir tree and its epiphytes: biomass, surface area, and their distribution. Can. J. For. Res.7, 680–699 (1977)

    Google Scholar 

  33. Ruinen, J.: Nitrogen fixation in the phyllosphere. In: The Biology of Nitrogen Fixation, edited by A. Quispel, pp. 121–167. North-Holland Publishing Co., Amsterdam (1974)

    Google Scholar 

  34. Sands, D. C., and A. D. Rovira: Isolation of fluorescent pseudomonads with a selective medium. Appl. Microbiol.20, 513–514 (1970)

    Google Scholar 

  35. Sherwood, M., and G. C. Carroll: Fungal succession on needles and young twigs of old-growth Douglas fir. Mycologia66, 499–506 (1974)

    Google Scholar 

  36. Spalding, B.: Enzymatic activities related to the decomposition of coniferous leaf litter. Soil Sci. Soc. Am. J.41, 622–627 (1977)

    Google Scholar 

  37. Tabatabai, M. A., and J. M. Bremner: Arylsulfatase activity of soils. Soil Sci. Soc. Am. Proc.34, 225–229 (1970)

    Google Scholar 

  38. Wright, E., and W. B. Bollen: Microflora of Douglas fir forest soil. Ecology42(4), 825–828 (1961)

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, Oregon State University, 97331, Corvallis, Oregon

    B. A. Caldwell, C. Hagedorn & W. C. Denison

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  1. B. A. Caldwell
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  2. C. Hagedorn
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  3. W. C. Denison
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Caldwell, B.A., Hagedorn, C. & Denison, W.C. Bacterial ecology of an old-growth douglas fir canopy. Microb Ecol 5, 91–103 (1979). https://doi.org/10.1007/BF02010500

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