Abstract
The effect of thiosulfate on dark assimilation of carbon dioxide in shallow marine environments was investigated in order to explain the recent discovery of bacterial thiosulfate oxidation in aerobic, open ocean seawater. The results demonstrate that the potential exists for microbial thiosulfate oxidation to increase both dark assimilation of carbon dioxide and the utilization of organic compounds in the sea. Thiosulfate-stimulated microbial activity may be caused not only by chemoautotrophic sulfur bacteria, but also by heterotrophic species which oxidize thiosulfate to tetrathionate. Measurements of dark assimilation of carbon dioxide made at different incubation times indicate that great care must be taken both in experimental procedure and in interpretation of results obtained with the dark assimilation technique.
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References
Albright, L. J.: The influence of hydrostatic pressure upon biochemical activities of heterotrophic bacteria.Can. J. Microbiol. 21, 1406–1412 (1975)
Barnes, H.: Apparatus and Methods of Oceanography, Part One: Chemical. Interscience Publishers, Inc., New York (1959)
Belly, R. T., and T. D. Brock: Ecology of iron-oxidizing bacteria in pyritic materials associated with coal.J. Bacteriol. 117, 726–732 (1974)
Eppley, R. W., and J. H. Sharp: Photosynthetic measurements in the central North Pacific: the dark loss of carbon in 24-h incubations.Limnol. Oceanogr. 20, 981–987 (1976)
Fliermans, C. B., and T. D. Brock: Ecology of sulfur-oxidizing bacteria in hot acid soils.J. Bacteriol. 111, 343–350 (1972)
Hicks, S. E., and F. G. Carey: Glucose determination in natural water.Limnol. Oceanogr. 13, 361–363 (1968)
Kelley, D. P., L. A. Chambers, and P. A. Trudinger: Cyanolysis and spectrophotometric estimation of trithionate in mixture with thiosulfate and tetrathionate.Anal. Chem. 41, 898–901 (1970)
Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall: Protein measurement with the folin phenol reagent.J. Biol. Chem. 193, 265–275 (1951)
Nalewajko, C., T. G. Dunstall, and H. Shear: Kinetics of extracellular release in axenic algae and in mixed algal-bacterial cultures: significance in estimation of total (gross) phytoplankton excretion rates.J. Phycol. 12, 1–5 (1976)
Roy, A. B., and P. A. Trudinger: The Biochemistry of Inorganic Compounds of Sulphur. Cambridge University Press, London (1970)
Santer, M., J. Boyer, and U. Santer:Thiobacillus novellus. 1. Growth on organic and inorganic media.J. Bacteriol. 78, 197–202 (1959)
Seki, H.: Relationship between production and mineralization of organic matter in Aburatsubo Inlet, Japan.J. Fish. Res. Bd. Can. 25, 625–637 (1968)
Sen Gupta, R., and H. W. Jannasch: Photosynthetic production and dark-assimilation of CO2 in the Black Sea.Int. Rev. Ges. Hydrobiol. 58, 625–632 (1973)
Steeman Nielsen, E.: Dark fixation of CO2 and measurements of organic productivity with remarks on chemo-synthesis.Physiol. Plant. 13, 348–357 (1960)
Sorokin, Yu. I.: A quantitative study of the microflora in the Central Pacific Ocean.J. Cons. Perm. Int. Explor. Mer. 29, 25–40 (1964)
Sorokin, Yu. I.: On the primary production and bacterial activities in the Black Sea.J. Cons. Perm. Int. Explor. Mer. 29, 41–60 (1964)
Sorokin, Yu. I.: On the trophic role of chemosynthesis in water bodies.Int. Rev. Ges. Hydrobiol. 49, 307–324 (1964)
Sorokin, Yu. I.: Experimental investigation of the rate and mechanism of oxidation of hydrogen sulfide in the Black Sea using S-35.Oceanology 10, 37–46 (1970)
Sorokin, Yu. I.: On the role of bacteria in productivity of tropical oceanic waters.Int. Rev. Ges. Hydrobiol. 56, 1–48 (1971)
Sorokin, Yu. I.: The bacterial population and the process of hydrogen sulfide oxidation in the Black Sea.J. Cons. Perm. Int. Explor. Mer. 34, 423–454 (1972)
Taguchi, S., and T. Platt: Assimilation of14CO2 in the dark compared to phytoplankton production in a small coastal inlet.Estuar. Costal Mar. Sci. (in press)
Tuttle, J. H., P. E. Holmes, and H. W. Jannasch: Growth rate stimulation of marine pseudomonads by thiosulfate.Arch. Microbiol. 99, 1–14 (1974)
Tuttle, J. H., P. E. Holmes, and H. W. Jannasch: Growth stimulation by thiosulfate in marine pseudomonads. Abstracts of the Annual Meeting of the American Society for Microbiology, p. 59 (1974)
Tuttle, J. H., and H. W. Jannasch: Occurrence and types of thiobacillus-like bacteria in the sea.Limnol. Oceanogr. 17, 532–543 (1972)
Tuttle, J. H., and H. W. Jannasch: Sulfide and thiosulfate-oxidizing bacteria in anoxic marine basins.Mar. Biol. 20, 64–70 (1973)
Tuttle, J. H., and H. W. Jannasch: Dissimilatory reduction of inorganic sulfur by facultatively anaerobic marine bacteria.J. Bacteriol. 115, 732–737 (1973)
Tuttle, J. H., and H. W. Jannasch: Microbial utilization of thiosulfate in the deep sea.Limnol. Oceanogr. 21, 697–701 (1976)
Vishniac, W. V., and M. Santer: The thiobacilli.Bacteriol. Rev. 21, 195–214 (1957)
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Tuttle, J.H., Jannasch, H.W. Thiosulfate stimulation of microbial dark assimilation of carbon dioxide in shallow marine waters. Microb Ecol 4, 9–25 (1977). https://doi.org/10.1007/BF02010426
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DOI: https://doi.org/10.1007/BF02010426