Abstract
Growth plates of rat bones have been studied with the electron microscope. Autoradiography of these tissues was made on specimens obtained at 6, 15, 30, 60, 180, 360, and 1200 min following intraperitoneal injection of45Ca. Isotopic45Ca was first noted in relation to the cells. Cells from the zones of proliferation, matrix synthesis, and hypertrophy contained isotopic calcium in the cytoplasmic areas of both the ER and Golgi membranes. Cells of the hypertrophic zone and those of the zone of matrix mineralization showed the label to be associated principally with the widely distributed membranes and processes of cells in varying states of dissolution. The label was then observed within the matrix of the mineralizing globules. These data suggest that the cartilage cell acts as a calcium binding and concentrating system and that the calcium is ultimately used in the process of calcification. The membranes involved in calcium binding are those reported by other workers to be associated with ATP, mucopolysaccharide, and phosphatases. It might be suggested, therefore, that these cell membranes may be the site of primary nucleation.
Résumé
Des cartilages de conjugaison d'os de rats ont été étudiés au microscope électronique. L'autoradiographie de ces tissus est effectuée sur des spécimens obtenus 6, 15, 30, 60, 180, 360 et 1200 minutes après injection intrapéritonéale de45Ca. L'isotope45Ca est d'abord observé dans les cellules. Les cellules de la zone de prolifération, de la synthèse matricielle et du cartilage hypertrophique contiennent du calcium radio-actif au niveau du réticulum endoplasmique et de l'appareil de Golgi. Les cellules de la zone hypertrophique et celles de la zone de minéralisation contiennent l'isotope surtout au niveau des membranes et des prolongements cellulaires dans diverses conditions de dissolution. Le marquage apparait ensuite au niveau de la matrice de régions globulaires en voie de minéralisation. Il semble que la cellule cartilagineuse se comporte comme un système qui concentre et effectue les liaisons du calcium. Le calcium participe finalement à la calcification. Les membranes intervenant dans les liaisons calciques sont celles qui, selon d'autres auteurs, sont associées à l'ATP, les mucopolysaccharides et les phosphatases. Il se peut que ces membranes cellulaires soient à l'origine des premiers phénomènes de nucléation.
Zusammenfassung
Wachstumsscheiben von Rattenknochen wurden mit dem Elektronenmikroskop untersucht. Jeweils 6, 15, 30, 60, 180, 360 und 1200 min nach intraperitonealer Injektion von45Ca wurde von jeder Probe eine Autoradiographie dieser Gewebe ausgeführt. Das Isotop45Ca wurde zuerst in Beziehung zu den Zellen gebracht. Zellen der Entwicklungszonen, der Matrixsynthese- und der Hypertrophiegebiete enthielten isotopisches Calcium in den cytoplasmischen Flächen des endoplasmatischen Reticulums, sowie der Golgi-Membranen. Zellen aus hypertrophischen Gebieten und solche aus Zonen der Matrixmineralisation zeigten, daß die Markierung speziell mit den weit verbreiteten Membranen und mit den Vorgängen in den Zellen während der verschiedenen Auflösungsstadien verbunden ist. Die Markierung wurde dann in der Matrix der mineralisierenden Zentren beobachtet. Daraus wurde angenommen, daß die Knochenzellen als ein calciumbindendes und calciumkonzentrierendes System wirken und das Calcium schließlich im Verkalkungsprozeß gebraucht wird. Die an der Calciumbindung beteiligten Membranen sind diejenigen, welche nach Angaben anderer Autoren mit ATP, Mucopolysacchariden und Phosphatasen in Beziehung stehen. Demnach könnte angenommen werden, daß diese Zellmembranen den Ort der ersten Nucleation darstellen.
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This work was supported in part by Grant from Schering Corporation, and the Medical Research Foundation of Texas.
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Matthews, J.L., Martin, J.H., Lynn, J.A. et al. Calcium incorporation in the developing cartilaginous epiphysis. Calc. Tis Res. 1, 330–336 (1967). https://doi.org/10.1007/BF02008105
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DOI: https://doi.org/10.1007/BF02008105