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Electrophoretic and serological comparisons of pathogenesis-related (b) proteins from different plant species

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Abstract

Preparations of pathogenesis-related (b) proteins (PRs) from differentNicotiana species, tomato,Gynura aurantiaca, bean, and cowpea were compared to each other and to bean chitinase and a constitutive apple agglutinin by electrophoresis in polyacrylamide gels both in the absence and in the presence of SDS, and by serological double diffusion analysis using antisera against tobacco PRs and bean chitinase. PRs from different plant genera displayed a similar but not identical range of relative mobilities in both native and SDS gels, whereas bean chitinase and apple agglutinin were clearly different. None of the antisera reacted with any of the PR preparations from plant genera other than the one from which the antigen(s) had been derived. Whilst PRs within the genusNicotiana are serologically related and can be identical, PRs from different plant genera seem to be sufficiently different to be considered as genus-specific.

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Authors and Affiliations

  1. Department of Plant Physiology, Agricultural University, Arboretumlaan 4, 6703 BD, Wageningen, the Netherlands

    L. C. van Loon

  2. Station d'Amélioration des Plantes, INRA, B.V. 1540, 21034, Dijon Cedex, France

    S. Gianinazzi & P. Ahl

  3. Plant Pathology Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK

    R. F. White

  4. Laboratoire de Pathologie végétale, Faculté des Sciences agronomiques, 5800, Gembloux, Belgium

    Y. Abu-Jawdah

  5. Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK

    J. F. Antoniw

  6. Botanisches Institut der Universität Basel, Schönbeinstrasse 6, 4056, Basel, Switzerland

    T. Boller

  7. Max-Planck-Institut für Biochemie, D-8033, Planegg-Martinsried bei München, F.R. Germany

    A. Camacho-Henriquez

  8. Cátedra de Bioquímica y Química Agrícola, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica, Camino de Vera 14, Valencia-22, Spain

    V. Conejero

  9. Institut de Tabac, Domaine La Tour, B.P. 168, 24106, Bergerac, France

    J. C. Coussirat

  10. Department of Plant Pathology, University of Missouri, 108 Waters Hall, 65211, Columbia, MO, USA

    R. N. Goodman

  11. Institut für Pflanzenkrankheiten und Pflanzenschutz, Universität Hannover, Herrenhäuser Strasse 2, D-3000, Hannover 21, F.R. Germany

    E. Maiss

  12. Istituto di Fitovirologia Applicata del CNR, Via O. Vigliani 104, 10135, Torino, Italy

    P. Redolfi

  13. Department of Virus Research, John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK

    T. M. A. Wilson

Authors
  1. L. C. van Loon
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  2. S. Gianinazzi
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  3. R. F. White
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  4. Y. Abu-Jawdah
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  5. P. Ahl
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  6. J. F. Antoniw
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  7. T. Boller
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  8. A. Camacho-Henriquez
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  9. V. Conejero
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  10. J. C. Coussirat
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  11. R. N. Goodman
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  12. E. Maiss
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  13. P. Redolfi
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  14. T. M. A. Wilson
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  15. Y. A. M. Gerritsen
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  16. H. J. Van Telgen
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  17. E. J. Van Der Zaal
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van Loon, L.C., Gianinazzi, S., White, R.F. et al. Electrophoretic and serological comparisons of pathogenesis-related (b) proteins from different plant species. Netherlands Journal of Plant Pathology 89, 293–303 (1983). https://doi.org/10.1007/BF01995264

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  • DOI: https://doi.org/10.1007/BF01995264

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