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Neutrophil collagenase activation: The role of oxidants and cathepsin G

  • Inflammatory Cells
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Abstract

The events leading to neutrophil collagenase activationin vivo were analyzed using phorbol myristate acetate (PMA) stimulated neutrophil supernatant. Under the conditions when this supernatant was incubated with the serine proteinase inhibitor, phenylmethylsulfonyl fluoride (PMSF), and then treated with the oxidant, hypochlorous acid (HOCl), collagenase was activated. When cathepsin G, a known activator of neutrophil collagenase, was also present, less HOCl was required to activate the latent collagenase. These experiments support the conclusion that activation of neutrophil collagenase occursin vivo by both an oxidant and an enzymatic mechanism where the effectiveness of oxidants is enhanced by cathepsin G.

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Authors and Affiliations

  1. Biochemistry Department, UMDNJ-Robert Wood Johnson Medical School, 675 Hoes Lane, 08854, Piscataway, NJ, USA

    C. Capodici & R. A. Berg

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  1. C. Capodici
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  2. R. A. Berg
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Capodici, C., Berg, R.A. Neutrophil collagenase activation: The role of oxidants and cathepsin G. Agents and Actions 34, 8–10 (1991). https://doi.org/10.1007/BF01993223

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  • DOI: https://doi.org/10.1007/BF01993223

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