Abstract
A combination of65Zn-tracer determinations, oxidative analyses for glycerol, and a bioassay for uncomplexed Zn2+ have shown that: (i) zinc monoglycerolate (ZMG) dissolves in aqueous salt dissolutions/physiological media by dissociation into zinc ions and glycerol, but the rate and extent of ZMG dissolution depend upon pH, and/or concentration and complexing efficiency of zinc-ligands; (ii) under physiological conditions certain ligands present in skin and blood (e.g. citrate, lactate, albumin, histidine, glutathione and other thiols and, to a lesser extent, amino acids) accelerate ZMG dissolution; and (iii) there is a general correlation between the conditional stability constants (pH 7.3, 25°C) of zinc-ligand complexes and the ability of given ligands to (a) solubilize ZMGin vitro and (b) mask the irritancy of Zn2+ in vivo. These observations indicate a mechanism for the transformation of ZMG applied transdermally or subcutaneously, to bioactive zinc (anti-arthritic nutritional supplement, etc.).
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Abbreviations
- BSA:
-
Bovine serum albumin
- Hepes:
-
N-[2-Hydroxyethyl] piperazine-N 1[2-ethanesulfonic acid]
- JCPDS:
-
Joint Committe of Powder Diffraction Studies (USA)
- Mes:
-
2-[N-Morpholino] ethanesulfonic acid
- Pipes:
-
Piperazine-N,N 1 bis[2-ethanesulfonic acid]
- ZMG:
-
Zinc monoglycerolate
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Fairlie, D.P., Whitehouse, M.W. & Taylor, R.M. Zinc monoglycerolate — A slow-release source of therapeutic zinc: Solubilization by endogenous ligands. Agents and Actions 36, 152–158 (1992). https://doi.org/10.1007/BF01991243
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DOI: https://doi.org/10.1007/BF01991243