Abstract
The continuous dielectrophoresis of living cells is described. The technique uses stream-centered transport of suspended microorganisms through an especially shaped non-uniform electric field. The cells can be given a positive or negative displacement, i.e., can be pushed into or out of the region of higher field intensity, depending upon the frequency of the applied ac field, and upon the relative permittivities of the cells and the suspending medium. Yeast (Saccharomyces cerevisiae) and algal cells (Chlorella vulgaris) were found to provide spectra of dielectrophoretic responses varying with the applied frequency (10 to 600 kHz) and conductivity.
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References
Black, B. C.; Hammond, E. G. 1965a. J. Am. Oil Chem. Soc.42, 931.
Black, B. C.; Hammond, E. G. 1965b. J. Am. Oil Chem. Soc.42, 936.
Chen, C. S.; Pohl, H. A. 1974. Trans. N. Y. Acad. Sci.238, 176.
Crane, J. S. 1970. "The Dielectrophoresis of Cells", Ph.D. Dissertation, Oklahoma State University.
Crane, J. S.; Pohl, H. A. 1978. J. Biol. Phys6, 45.
Jones, T. B.; Bliss, G. W. 1977. J. Appl. Phys.48, 1412.
Mason, B. D.; Townsley, P. M. 1971. Can. J. Microbiol.17, 879.
Nichols, H. Wayne. 1973. "Growth media—fresh water", in HANDBOOK OF PHYCOLOGICAL METHODS (Janet R. Stein, ed). Cambridge University Press, Cambridge, p. 15.
Parmar, D. S.; Jalaluddin, A. K. 1974. Jap. J. Appl. Phys.13, 793.
Pohl, H. A. 1951. J. Appl. Phys.22, 869.
Pohl, H. A. 1958. J. Appl. Phys.29, 1182.
Pohl, H. A. 1960. Sci. Am.203, 107.
Pohl, H. A. 1968. J. Electrochem. Soc.115, 155c.
Pohl, H. A. 1969. In DIELECTROPHORETIC AND ELECTROPHORETIC SEPARATION, (H. A. Pohl and W. F. Pickard, eds.). The Electrochemical Society, New York, p. 12.
Pohl, H. A.; Crane, J. S. 1971. Biophys. J.11, 711.
Pohl, H. A.; Crane, J. S. 1972. J. Theor. Biol.37, 1.
Pohl, H. A. 1973. In ELECTROSTATICS AND ITS APPLICATIONS, (A. D. Moore, ed.). Wiley-Interscience, N.Y., pp. 342–3.
Pohl, H. A.; Crane, J. S. 1973. In ELECTROSTATICS AND ITS APPLICATIONS, (A. D. Moore, ed.). Wiley-Interscience, N.Y. pp. 368–70.
Pohl, H. A.; Pethig, R. 1977. J. Phys. E.10, 190.
Pohl, H. A. 1978. DIELECTROPHORESIS. Cambridge University Press, Cambridge, England.
Rhoads, J. E.; Pohl, H. A.; Buckner, R. G. 1976. J. Biol. Phys.4, 93.
Sher, L. 1968. Nature (London)220, 695.
Ting, I. P.; Jolley, K.; Beasley, C. A.; Pohl, H. A. 1971. Biochim. Biophys. Acta234, 324.
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Pohl, H.A., Kaler, K. & Pollock, K. The continuous positive and negative dielectrophoresis of microorganisms. J Biol Phys 9, 67–86 (1981). https://doi.org/10.1007/BF01987284
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DOI: https://doi.org/10.1007/BF01987284