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A sum of14C-putrescine metabolites as a measure of DAO activity. Column chromatography assay

  • Metabolism of Histamine
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Abstract

The rapid ion-exchange chromatographic method was developed for the separation of14C-putrescine from its metabolites arising from diamine oxidase (DAO, E.C. 1.4.3.6) catalysed reaction. The assay is linear for putrescine conversion. The assay is linear for putrescine conversion with both, time and enzyme concentration. With this method many samples could be analysed simultaneously.

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Authors and Affiliations

  1. Department of Biogenic Amines, Polish Academy of Sciences, Narutowicza 60, 90 136, Lodz, Poland

    W. A. Fogel, M. Ulatowska, K. Adach & Z. Osińska

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  1. W. A. Fogel
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  2. M. Ulatowska
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  3. K. Adach
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  4. Z. Osińska
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Fogel, W.A., Ulatowska, M., Adach, K. et al. A sum of14C-putrescine metabolites as a measure of DAO activity. Column chromatography assay. Agents and Actions 16, 99–101 (1985). https://doi.org/10.1007/BF01983111

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  • DOI: https://doi.org/10.1007/BF01983111

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