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Serum antibody response to filamentous hemagglutinin in patients with clinical pertussis measured by an enzyme-linked immunosorbent assay

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Abstract

Titers of antibodies to filamentous hemagglutinin (FHA) were determined by enzymelinked immunosorbent assay in acute and convalescent phase serum samples from 158 patients with clinical symptoms typical of whooping-cough. In 96 of the patients the diagnosis was verified by culture. Significant changes in serum levels of IgG, IgM and/or IgA antibodies against FHA were demonstrated in 126 patients (80%). Thus, demonstration of significant changes in FHA antibody titers in serum can be used for serological diagnosis of pertussis. The results also show that high levels of IgG, IgM and/or IgA antibodies in a single serum sample suggest current pertussis infection, but if the diagnosis is based on determinations of FHA antibody titers in a single serum sample the sensitivity is low. The levels of antibody to FHA were compared with previously determined levels of antibodies to pertussis toxin. A significant antibody response against both FHA and pertussis toxin was seen in 111 patients (70 %) while 147 patients (93 %) developed a significant increase in antibodies against one or both antigens.

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References

  1. Kwantes, W., Joynson, D. H. M., Williams, W. O. Bordetella pertussis isolation in general practice: 1977–79 whooping cough epidemic in West Glamorgan. Journal of Hygiene 1983, 90: 149–158.

    PubMed  Google Scholar 

  2. Mertsola, J., Kuronen, T., Turunen, A., Viljanen, M. K., Ruuskanen, O. Diagnosis of pertussis. Journal of Infection 1984, 8: 149–156.

    PubMed  Google Scholar 

  3. Sato, Y., Cowell, J. L., Sato, H., Burstyn, D. G., Manclark, C. R. Separation and purification of the hemagglutinins fromBordetella pertussis. Infection and Immunity 1983, 41: 313–320.

    PubMed  Google Scholar 

  4. Zackrisson, G., Krantz, I., Lagergård, T., Larsson, P., Sekura, R., Sigurs, N., Taranger, J., Trollfors, B. Antibody response to pertussis toxin in patients with clinical pertussis measured by enzyme-linked immunosorbent assay. European Journal of Clinical Microbiology & Infectious Diseases 1988, 7: 149–154.

    Google Scholar 

  5. Keogh, E. V., North, E. A., Warburton, M. F. Hemagglutinins of theHaemophilus group. Nature 1947, 160: 63.

    Google Scholar 

  6. Granström, M., Lindberg, A. A., Askelöf, P., Hederstedt, B. Detection of antibodies in human serum against the fimbrial haemagglutinin ofBordetella pertussis by enzyme-linked immunosorbent assay. Journal of Medical Microbiology 1982, 15: 85–96.

    PubMed  Google Scholar 

  7. Irons, L. I., Ashworth, L. A. E., Wilton-Smith, P. Heterogeneity of filamentous haemagglutinin ofBordetella pertussis studied with monoclonal antibodies. Journal of General Microbiology 1983, 129: 2769–2778.

    PubMed  Google Scholar 

  8. Sato, Y., Izumiya, K., Sato, M., Cowell, J. L., Manclark, C. R. Role of antibody to leukocytosis-promoting factor hemagglutinin and to filamentous hemagglutinin in immunity to pertussis. Infection and Immunity 1981, 31: 1223–1231.

    PubMed  Google Scholar 

  9. Tuomanen, E., Weiss, A. Characterization of two adhesins ofBordetella pertussis for human ciliated respiratory-epithelial cells. Journal of Infectious Diseases 1985, 152: 118–125.

    PubMed  Google Scholar 

  10. Granström, M., Granström, G., Lindfors, A., Askelöf, P. Serologic diagnosis of whooping cough by an enzymelinked immunosorbent assay using fimbrial haemagglutinin as antigen. Journal of Infectious Diseases 1982, 146: 741–745.

    PubMed  Google Scholar 

  11. Håkansson, S., Sundin, C. G., Granström, M., Gästrin, B. Diagnosis of whooping cough — a comparison of culture, immunofluorescence and serology with ELISA. Scandinavian Journal of Infectious Diseases 1984, 16: 281–284.

    PubMed  Google Scholar 

  12. Askelöf, P., Granström, M., Gillenius, P., Lindberg, A. A. Purification and characterisation of a fimbrial haemagglutinin fromBordetella pertussis for use in an enzyme-linked immunosorbent assay. Journal of Medical Microbiology 1982, 15: 73–83.

    PubMed  Google Scholar 

  13. Zackrisson, G., Lagergård, T., Lönnroth, I. An enzyme-linked immunosorbent assay method for detection of immunoglobulins to pertussis toxin. Acta Pathologica et Microbiologica Scandinavica, (C) 1986, 94: 227–231.

    Google Scholar 

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Authors and Affiliations

  1. Department of Clinical Bacteriology, University of Göteborg, Göteborg, Sweden

    G. Zackrisson

  2. Institut Mérieux, Marcy l'Etoile, France

    F. Arminjon

  3. Department of Infectious Diseases, University of Göteborg, Göteborg, Sweden

    I. Krantz

  4. Institute of Medical Microbiology, University of Göteborg, Göteborg, Sweden

    T. Lagergård

  5. Department of Pediatrics, Borås Hospital, Borås, Sweden

    N. Sigurs

  6. Pediatric Out-patient Clinic, Västra Frölunda Hospital, Västra Frölunda, Sweden

    J. Taranger

  7. Department of Infectious Diseases, University of Umeå, Umeå, Sweden

    B. Trollfors

Authors
  1. G. Zackrisson
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  2. F. Arminjon
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  3. I. Krantz
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  4. T. Lagergård
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  5. N. Sigurs
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  6. J. Taranger
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  7. B. Trollfors
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Zackrisson, G., Arminjon, F., Krantz, I. et al. Serum antibody response to filamentous hemagglutinin in patients with clinical pertussis measured by an enzyme-linked immunosorbent assay. Eur. J. Clin. Microbiol. Infect. Dis. 7, 764–770 (1988). https://doi.org/10.1007/BF01975044

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  • DOI: https://doi.org/10.1007/BF01975044

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