Abstract
A novel method for the production of basidiocarps from the mycelia ofCrinipellis perniciosa is described. This involved the colonization of a bran-vermiculite medium with pure culture of the fungus, prior to application of a peat-based casing. Basidiocarp production was induced by hanging the cultures in a cabinet where they were subjected to a daily cycle of wetting and drying. The method was successfully and reproducibly used to fruit isolates of all four known biotypes of the fungus within 10–16 weeks of inoculation.
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References
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Griffith, G.M., Hedger, J.N. A novel method for producing basidiocarps of the cocoa pathogen Crinipellis perniciosa using a bran-vermiculite medium. Netherlands Journal of Plant Pathology 99, 227–230 (1993). https://doi.org/10.1007/BF01974667
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DOI: https://doi.org/10.1007/BF01974667