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Routine laboratory diagnosis of continuous ambulatory peritoneal dialysis peritonitis using centrifugation/lysis and saponin-containing media

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Abstract

This study examines the application in a routine laboratory of a method for concentrating and lysing leucocytes in continuous ambulatory peritoneal dialysis (CAPD) effluent to diagnose peritonitis. Between November 1986 and April 1990 pathogens from 42 of 82 (51 %) CAPD specimens from patients with acute peritonitis failed to grow on horse blood agar (HBA) after direct inoculation. Recovery rates of pathogens were 74 % (6 of 82) from a solid medium containing saponin and 89 % (73 of 82) after lysing the leucocytes before inoculating HBA. Microbial sequestration in leucocytes was demonstrated in 73% (60 of 82) of specimens, while pathogens from 11 % (9 of 82) were grown only from a liquid medium. Thirteen of 17 specimens with<500 leucocytes/mm3 grew on all three solid media. Gram stain microscopy revealed organisms in 51 % (41 of 81) of specimens. The method, using apparatus and media prepared for everyday examination of specimens, is sensitive, specific and yields single colonies of most species within 24 h.

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Authors and Affiliations

  1. Department of Microbiology, The Prince of Wales Hospital, 2031, Randwick, New South Wales, Australia

    P. C. Taylor

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  1. P. C. Taylor
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Taylor, P.C. Routine laboratory diagnosis of continuous ambulatory peritoneal dialysis peritonitis using centrifugation/lysis and saponin-containing media. Eur. J. Clin. Microbiol. Infect. Dis. 13, 249–252 (1994). https://doi.org/10.1007/BF01974545

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  • DOI: https://doi.org/10.1007/BF01974545

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