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Soybean trypsin inhibitor. An IL-1-like protein?

  • Cytokines
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Abstract

Soybean trypsin inhibitor (SBTI) shares some structural homology with interleukin-1 (IL-1) and was tested for IL-1 bioactivity. Human T-cells proliferated maximally when stimulated with PMA and SBTI but failed to respond to either stimulus alone. This response was abrogated by neutralizing antibodies to IL-1 beta but not to IL-1 alpha. However, immunoblots showed no cross-reactivity between SBTI and anti-IL-1 antibodies. Furthermore, SBTI did not bind to IL-1 receptors on YT cells and did not activate a murine T-lymphoma or human T-hybridoma. Supernantants from monocytes stimulated with SBTI contained significant levels of IL-1 activity. The data show that SBTI has no direct IL-1 activity but can stimulate T-cells indirectly through an IL-1 dependent mechanism.

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Authors and Affiliations

  1. Department of Hypersensitivity Diseases Cell Biology, The Upjohn Company, 49001, Kalamazoo, MI, USA

    A. E. Berger

  2. Department of Biopolymer Chemistry, The Upjohn Company, 49001, Kalamazoo, MI, USA

    M. R. Deibel Jr.

  3. Department of Physical and Analytical Chemistry Research, The Upjohn Company, 49001, Kalamazoo, MI, USA

    K. A. Richard, S. C. Speziale, N. D. Staite, B. C. Finzel & H. M. Einspahr

Authors
  1. K. A. Richard
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  2. S. C. Speziale
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  3. N. D. Staite
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  4. A. E. Berger
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  5. M. R. Deibel Jr.
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  6. B. C. Finzel
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  7. H. M. Einspahr
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Richard, K.A., Speziale, S.C., Staite, N.D. et al. Soybean trypsin inhibitor. An IL-1-like protein?. Agents and Actions 27, 265–267 (1989). https://doi.org/10.1007/BF01972792

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  • DOI: https://doi.org/10.1007/BF01972792

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