Abstract
We have previously described the isolation and initial characterization of 15 kDa protein isoforms (p15s) from rabbit polymorphonuclear leukocytes (PMN) that bind toEscherichia coli and modulate the antibacterial actions of other leukocyte proteins on this gram negative organism. We now report that the p15s differ in primary structure. The cloning and sequencing of two distinct p15 cDNAs from a rabbit bone marrow library reveal that two of the isoforms are closely similar in primary structure differing at only two amino acid positions. The p15 cDNAs encode putative signal sequences suggesting a granule-associated localization for these proteins. Analysis of the derived p15 primary structures reveals homology to two leukocyte proteins: CAP-18, an 18 kD lipopolysaccharide (LPS) binding protein from rabbit PMN and cathelin, an 11 kD cysteine protease inhibitor from porcine leukocytes. This structural similarity suggests the existence of a novel family of low molecular weight leukocyte proteins with potential roles in inflammation.
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References
P. Elsbach and J. Weiss, In Inflammation, (Eds. J. Gallin, I. M. Goldstein and R. Snyderman) 2nd Edition, Raven Press, New York (in press).
C. E. Ooi, J. Weiss, O. Levy and P. Elsbach,Isolation of two isoforms of a novel 15 kDa protein from rabbit polymorphonuclear leukocytes that modulate the antibacterial actions of other leukocyte proteins. J. Biol. Chem.265, 15956–15962 (1990).
F. Sanger, S. Nicklen and A. R. Coulson,DNA Sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA74, 5463–5467 (1977).
J. Devereux, P. Haeberli and O. Smithies,A comprehensive set of sequence analysis programs for the VAX. Nuc. Ac. Res.12, 387–395 (1984).
M. Kozak,The scanning model of translation: an update. J. Cell Biol.108, 229–241 (1989).
A. Ritonja, M. Kopitar, R. Jerala and V. Turk,Primary structure of a new cysteine proteinase inhibitor from pig leucocytes. Biochem. Biophys. Hopp. Sey.370, 1145–1151 (1989).
J. W. Larrick, J. G. Morgan, I. Palings, M. Hirata and M. H. Yen,Complementary DNA sequence of rabbit CAP-18- a unique lipopolysaccharide binding protein. Biochem. Biophys. Res. Commun.179, 170–175 (1991).
R. W. Colman,The role of plasma proteases in septic shock. N. Engl. J. Med.320, 1207–1209 (1989).
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Levy, O., Weiss, J., Ooi, C.E. et al. Structural characterization of BPI-modulating 15 kDa proteins from rabbit polymorphonuclear leukocytes: Identification of a novel family of leukocyte proteins. Agents and Actions 39 (Suppl 1), C207–C210 (1993). https://doi.org/10.1007/BF01972768
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DOI: https://doi.org/10.1007/BF01972768