Abstract
Guinea-pig peritoneal exudate cells, during in vitro culture, produce substantial concentrations of E-type prostaglandins (PGs) which are known to affect lymphocyte function. This suggests that PGs may act as regulators of lymphocyte responses.
Lymph node lymphocytes from animals exhibiting delayed hypersensitivity to purified protein derivative of tuberculin or protein antigen (bovine gamma globulin) were incubated, in serum-supplemented Eagles minimal essential medium, with specific antigen for 24 hours at 37°C. Lymphokines produced by such populations exhibit macrophage migration inhibition, lymphocyte mitogenicity, and produce increased vascular permeability. When PGE1 or PGE2 (0.01–1.0 μg/ml) are included in the incubation medium, they inhibit the production of macrophage migration inhibitory and lymphocyte mitogenic activities, but enhance production of the inflammatory lymphokine activity. These observations are consistent with the suppressive action of PGE on in vitro analogues of delayed hypersensitivity, and with the stimulatory action of PGE on in vivo vascular manifestations of delayed hypersensitivity. It has been observed that lymphokine preparations produced by peritoneal exudate cells exhibit marked heterogeneity with respect to these three activities, and PGE production by macrophages in such cell populations may contribute to this heterogeneity.
The differential action of E-type PGs on the secretion of different lymphokine activities implies that these activities are due to distinct substances, and, moreover, suggests that different lymphokine activities may originate from distinct subpopulations of lymphocytes.
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Bray, M.A., Gordon, D. & Morley, J. Regulation of lymphokine secretion by prostaglandins. Agents and Actions 6, 171–175 (1976). https://doi.org/10.1007/BF01972203
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DOI: https://doi.org/10.1007/BF01972203