Abstract
High-field13C-NMR of13C-enriched compounds in dilute aqueousd 6-Me2SO solutions provides a simple, accurate method for measuring pK as of sparingly soluble carboxylic acids. Using this method, we found the pK as of mesobilirubin-XIIIα to be 4.2 and 4.9, much lower values than reported recently for bilirubin, and of mesobiliverdin-XIIIα to be 3.9 and 5.3.
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Lightner, D.A., Holmes, D.L. & McDonagh, A.F. Dissociation constants of water-insoluble carboxylic acids by13C-NMR. pK as of mesobiliverdin-XIIIα and mesobilirubin-XIIIα. Experientia 52, 639–642 (1996). https://doi.org/10.1007/BF01969747
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DOI: https://doi.org/10.1007/BF01969747