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A simple method of DNA extraction from whole tissues and blood using glass powder for detection of transgenic animals by PCR

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Abstract

A very simple and reliable method to extract DNA directly from mouse tail, rabbit ear and blood is described. Tissue was homogenized in a solution of NaI and the DNA was extracted using glass powder. The extracted DNA was obtained in sufficient quantity and purity to allow direct detection of transgene by PCR.

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Authors and Affiliations

  1. Unité de Differentiation Cellulaire, Institut National de la Recherche Agronomique, 78352, Jouy-en-Josas Cedex, France

    Joe Attal, Marco Cajero-Juarez & Louis-Marie Houdebine

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  1. Joe Attal
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  2. Marco Cajero-Juarez
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  3. Louis-Marie Houdebine
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Attal, J., Cajero-Juarez, M. & Houdebine, LM. A simple method of DNA extraction from whole tissues and blood using glass powder for detection of transgenic animals by PCR. Transgenic Research 4, 149–150 (1995). https://doi.org/10.1007/BF01969417

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  • DOI: https://doi.org/10.1007/BF01969417

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