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A modified radioimmunoassay for the detection of histamine releasein vivo andin vitro in man

  • Histamine: Measurement and Clinical Studies
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Abstract

Histamine (Hi) releasein vitro orin vivo in man was measured by variants of a radioimmunoassay (RIA) procedure. Hi released from isolated basophils was converted enzymically to N-τ-methylhistamine (NMH) which was then measured by a very sensitive RIA. This modified RIA was compared with the standard spectrofluorometric assay and was found to have additional advantages in certain applications. RIA of NMH in plasma was found to be of value in acute medical conditions of obscure aetiology.

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Authors and Affiliations

  1. Department of Pharmacology, University College London, Gower Street, WC1E 6BT, London, UK

    E. S. K. Assem & D. K. Osei

  2. University College Hospital, WC1, London, UK

    E. S. K. Assem & D. K. Osei

Authors
  1. E. S. K. Assem
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  2. D. K. Osei
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Assem, E.S.K., Osei, D.K. A modified radioimmunoassay for the detection of histamine releasein vivo andin vitro in man. Agents and Actions 30, 287–290 (1990). https://doi.org/10.1007/BF01969063

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  • DOI: https://doi.org/10.1007/BF01969063

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