Abstract
The key-enzyme for the metabolism of diamines in man is diamine oxidase (DAO). Its highest activities are in the intestinal mucosa, localized in the cytoplasm of the mature enterocytes of the small and large bowel. If the gut is affected by inflammation in Crohn's disease macroscopical changes are observed. This prospective study investigated if these mucosal alterations are also reflected in changes of mucosal diamine oxidase activity and/or mucosal histamine content respectively. Twenty patients (12 female, 8 male; age:\(\tilde x = 31\), range 18 49 years) undergoing gut resection because of complications in Crohn's disease (Jan.–Dec. 1988) formed the basis of the study. Tissue samples of the resected material from areas inflamed and histologically not involved in the disease were investigated for diamine oxidase activities and histamine content. Diamine oxidase activities in the mucosa obtained from the macroscopically normal proximal (155.6; (76–393) mU/g (\(\tilde x\) range)) and distal (132; (58.5–295) mU/g) resection margins were similar to our previous findings. In all patients, however, samples from the diseased mucosa had significantly (ca. 50%) lower diamine oxidase activities (74.5; (5–262) mU/g) compared to the healthy tissue. Similar differences were found in material obtained either from whole intestinal wall or from the mucosa. The determination of diamine oxidase activity constitutes possibly a more unambiguous and earlier parameter for assessing the extent of the inflamed area than histological disease presentations. Using biopsies the necessary extent of resection could be estimatedbefore operation: this may influence operative strategies and help in the definition of the minimum amount of inflamed gut to be removed.
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References
A. M. Dvorak, A. B. Connell and G. R. Dickersin,Crohn's disease: A scanning electron microscopic study. Human Pathol.10, 165–177 (1979).
M. Wienbeck, J. Purrmann und B. Miller,Colitis ulcerosa und Morbus Crohn: Frühdiagnostik und frühzeitige Therapie. Internist27, 166–171 (1986).
Ch. Herfarth and Th. Heil,Morbus Crohn und Colitis ulcerosa-Standortbestimmung: Chirurgische Aspekte. Chirurg52, 749–757 (1981).
S. O. Ukabam, J. R. Clamp and B. T. Cooper,Abnormal small intestinal permeability to sugars in patients with Crohn's disease of the terminal ileum and colon. Digestion27, 70–74 (1983).
L. D'Agostino, B. Daniele, F. Pallone, S. Pignata, M. Leoni and G. Mazzacca,Postheparin plasma diamine oxidase in patients with small bowel crohn's disease. Gastroenterology95, 1503–1509 (1988).
J. S. Thompson, D. A. Burnett, R. A. Cormier and W. P. Vaughan,Plasma postheparin diamine oxidase activity-development of a simple technique of assessing Crohn's disease. Dis. Col. Rect.31, 529–532 (1988).
T. Bieganski, J. Kusche and W. Lorenz,Distribution and properties of human intestinal diamine oxidase and its relevance for the histamine catabolism. Biochem. Biophys. Acta756, 196–203 (1983).
K. M. Shakir, S. Margolis, S. B. Baylin,Localization of histaminase (diamine oxidase) in rat small intestinal mucosa: site of release by heparin. Biochem. Pharmacol.26, 2343–2347 (1977).
R. Hesterberg, J. Sattler, W. Lorenz, C. D. Stahlknecht, H. Barth, M. Crombach and D. Weber,Histamine content, diamine oxidase activity and histamine methyltransferase activity in human tissues: fact or fictions? Agent and Actions14, 325–334 (1984).
J. Kusche, H. Richter, R. Hesterberg, J. Schmidt and W. Lorenz,Comparison of the 14-C-putrescin-assay with the NADH-test for the determination of diamine oxidase: description of a standard procedure with a high precision and an improved accuracy. Agents and Actions3, 148–156 (1973).
W. Lorenz, K. Thon, E. Neugebauer, H. Stöltzing, Ch. Ohmann, D. Weber, A. Schmal, E. Hinterlang, H. Barth and J. Kusche,Reliability and practicability of the fluorometric-fluoroenzymatic histamine determination in pathogenetic studies on peptic ulcer: Detection limits and problems with specificity. Agents and Actions21, 1–25 (1987).
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Supported by grant of Deutsche Forschungsgemeinschaft (Lo 199/15-2).
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Schmidt, W.U., Sattler, J., Hesterberg, R. et al. Human intestinal diamine oxidase (DAO) activity in Crohn's disease: A new marker for disease assessment?. Agents and Actions 30, 267–270 (1990). https://doi.org/10.1007/BF01969057
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DOI: https://doi.org/10.1007/BF01969057