Skip to main content
SpringerLink
Log in

An improved spectrophotometric assay for histamine and diamine oxidase (DAO) activity

  • Histamine and Kinins
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

Diamine oxidase or its substrates can be measured using a coupled reaction with peroxidase and the chromogen 3-methyl-2-benzothiazolone hydrazone (MBTH) with an appropriate acceptor such as 3-(dimethylamino)benzoic acid (DMAB). This method provides a more rapid and sensitive method of measuring histamine than current colorimetric assays; using this system, histamine was quantitated at concentrations of 10 to 400 μmol/l. TheK m of DAO was determined to be 2.9×10−5 M, a lower value than previously reported.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C.H. Best,The disappearance of histamine from autolysing lung tissue, J. Physiol.67, 256 (1929).

    Google Scholar 

  2. E.A. Zeller,Uber den enzymatischen Abbau von Histamin und Diaminen, Helv. chim. Acta21, 880 (1938).

    Article  Google Scholar 

  3. C.H. Best andE.W. McHenry,The inactivation of histamine, J. Physiol.70, 1349 (1930).

    Google Scholar 

  4. E.A. Zeller, H. Birkhauser, H. Mislin andM. Wenk,Uber das Vorkommen der Diamin-oxydase bei Mensch, Saugetier und Vogel. Mit einem Anhang uber das Vorkommen der Cholin-esterase beim Vogel, Helv. chim. Acta22, 1381 (1939).

    Article  Google Scholar 

  5. B. Mondovi, P. Guerrieri, A.M. Wolf, A. Ballini andP. Gerosa,Properties of immobilized histaminase, Agents and Actions9, 40 (1979).

    PubMed  Google Scholar 

  6. W. Lorenz, J. Kusche, H. Hahn andE. Werle,Determination of the activity of diamine oxidases, urease, and histidine ammonia-lyase by enzymatic assay of ammonia, Z. analyt. Chem.243, 259 (1968).

    Article  Google Scholar 

  7. R. Kapeller-Adler andH. McFarlane,Purification and identification of hog-kidney histaminase, Biochim. biophys. Acta67, 543 (1963).

    Article  Google Scholar 

  8. P.N. Aarsen andA. Kemp,Rapid spectrophotometric micromethod for determination of histaminase activity, Nature1195 (1964).

  9. W.G. Bardsley, M.J.C. Crabbe, J.S. Shindler andJ.A. Ashford,Oxidation of p-Dimethylaminomethylbenzylamine by Pig Kidney Diamine Oxidase, Biochem. J.127, 875 (1972).

    PubMed  Google Scholar 

  10. M.F. Tourkof, G.I. Davydova, K.M. Yermolaev andV.Z. Gorkin,Colorimetric estimation of diamine oxidase activity, Analyt. Biochem.77, 68 (1977).

    Article  PubMed  Google Scholar 

  11. S. Dupre, S.P. Solinas, P. Guerrieri, G. Federici andD. Cavallini,NADH-coupled spectrophotometric assay of diamine oxidase, Analyt. Biochem.77, 68 (1977).

    Article  PubMed  Google Scholar 

  12. A.C. Andersson, S. Henningsson, L. Persson andE. Rosengren,Aspects of diamine oxidase activity and its determination, Acta physiol. scand.102, 159 (1978).

    PubMed  Google Scholar 

  13. E. Neufeld andR. Chayen,An evaluation of three methods for the measurement of diamine oxidase activity in amniotic fluid, Analyt. Biochem.96, 411 (1979).

    Article  PubMed  Google Scholar 

  14. E.A. Zeller,Diamine Oxidases, The Enzymes, Second edition, Vol. 8 (Ed.P. D. Boyer et al.) Academic Press, New York 1963.

    Google Scholar 

  15. J. Kusche andW. Lorenz,Diamine Oxidase Methods of Enzymatic Analysis, Vol. 3, p. 237 (EdH. U. Bergmeyer). Academic Press, New York 1983.

    Google Scholar 

  16. T.T. Ngo andH.M. Lenhoff,A sensitive and versatile chromogenic assay for peroxidase and peroxidase-coupled reactions, Analyt. Biochem.105 389 (1980).

    Article  PubMed  Google Scholar 

  17. E. Sawicki, T.W. Stanley, T.R. Hauser, W. Elbert andJ.L. Noe,Spot test detection and colorimetrion determination of aromatic amines and imino hetero aromatic compounds with 3-methyl-2-benzothiazolona hydrazone, Analyt. Chem.33, 722 (1961).

    Article  Google Scholar 

Download references

Author information

Author notes

  1. P. Stoner

    Present address: , Penna. 15530, Berlin

Authors and Affiliations

  1. Former Research Associate, Department of Biochemistry, University of Southern Mississippi, Mississippi, USA

    P. Stoner

Authors
  1. P. Stoner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stoner, P. An improved spectrophotometric assay for histamine and diamine oxidase (DAO) activity. Agents and Actions 17, 5–9 (1985). https://doi.org/10.1007/BF01966672

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01966672

Keywords

Search

Navigation