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LPS-stimulated bovine aortic endothelial cells produce IL-1 and IL-6 like activities

  • Inflammation and Immunomodulation
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Abstract

Vascular endothelium is known to closely interact with leukocytes and immunocompetent cells. We report here that cultured bovine aortic endothelial cells (BAEC) synthesize both interleukin 1 (IL-1) and interleukin 6 (IL-6) like activities in response to bacterial lipopolysaccharide stimulation. Our results agree with previous data obtained from human venous endothelia and support the concept that IL-1 and IL-6 synthesis are properties common to endothelial cells from different vascular beds. The IL-1 activity was measured by murine thymocyte proliferation assay and by an indirect bioassay using NOB1 cells, which evidenced higher IL-1 amounts than the former. This discrepancy appeared to be partly due to the simultaneous production of one or more inhibitor(s) of the thymocyte proliferation by BAEC. The IL-6 assay was performed with the murine hydridoma cell line B9. In other respects, the cyclooxygenase inhibitor indomethacin enhanced the IL-1 like production, but was ineffective on IL-6 like production. The present study provides additional evidence that endothelial cells from large arteries may also participate in inflammatory and immunological processes.

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Authors and Affiliations

  1. Laboratoire de Physiologie Cellulaire, Faculté de Pharmacie, Université de Montpellier I, 15, Avenue Charles Flahault, 34060, Montpellier Cedex, France

    G. Modat, D. Junquero, A. Mary, A. Muller & C. Bonne

  2. Laboratoire de Biologie Cellulaire, INSERM U 65, 34060, Montpellier Cedex, France

    J. Dornand & N. Bernad

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  1. G. Modat
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  2. J. Dornand
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  3. N. Bernad
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  5. A. Mary
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  6. A. Muller
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  7. C. Bonne
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Modat, G., Dornand, J., Bernad, N. et al. LPS-stimulated bovine aortic endothelial cells produce IL-1 and IL-6 like activities. Agents and Actions 30, 403–411 (1990). https://doi.org/10.1007/BF01966305

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