Skip to main content
SpringerLink
Log in

A new continuous optical assay for isocitrate lyase

  • Short Communications
  • Published:
Experientia Aims and scope Submit manuscript

Summary

A new continuous optical assay method for isocitrate lyase is reported. This is a coupled assay which requires lactate dehydrogenase as an ancillary enzyme. The method yields linear data up to 0.12 units/ml. The assay is also suitable for crude extracts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. McFadden, B.A., and Howes, W.V., Analyt. Biochem.1 (1960) 240.

    Article  Google Scholar 

  2. Dixon, G.H., and Kornberg, H.L., Biochem. J.72 (1959) 3P.

  3. Johanson, R.A., Hill, J.M., and McFadden, B.A., Biochim. biophys. Acta364 (1974) 327.

    PubMed  Google Scholar 

  4. Vincenzini, M.T., Pinzauti, G., and Vanni, P., It. J. Biochem.29 (1981) 329.

    Google Scholar 

  5. Pinzauti, G., Giachetti, E., and Vanni, P., Int. J. Biochem.14 (1982) 267.

    Article  PubMed  Google Scholar 

  6. Warren, W.A., J. biol. Chem.245 (1970) 1675.

    PubMed  Google Scholar 

  7. Roche, T.E., Williams, J.O., and McFadden, B.A., Biochim. biophys. Acta206 (1970) 193.

    PubMed  Google Scholar 

  8. Wilkinson, J.N., Biochem. J.80 (1961) 324.

    PubMed  Google Scholar 

  9. Cleland, W.W., Adv. Enzymol.29 (1967) 1.

    PubMed  Google Scholar 

  10. Segel, I.H., Enzyme kinetics. Wiley. New York 1975.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto interfacoltà di Chimica biologica, Università di Firenze, viale Morgagni 50, I-50134, Firenze, (Italy)

    E. Giachetti, G. Pinzauti & P. Vanni

Authors
  1. E. Giachetti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Pinzauti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Vanni
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Acknowledgment. This research was supported by grants of the Italian Ministero della Pubblica Istruzione.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Giachetti, E., Pinzauti, G. & Vanni, P. A new continuous optical assay for isocitrate lyase. Experientia 40, 227–228 (1984). https://doi.org/10.1007/BF01963614

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01963614

Keywords

Search

Navigation