Summary
Mouse morulae were frozen to −196°C in the presence of 1.2 M ethylene glycol or 1 M glycerol. After iceseeding in embryo samples at −10°C or below, the survival rates in vitro were lower than those obtained by ice-seeding between −4° and −8°C. The proportion morulae developing into live young in vivo after ice-seeding at −13°C and freezing was lower than that of embryos seeded at −4°C.
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Miyamoto, H., Ishibashi, T. Effects of the temperature of ice-seeding on survival of frozen-and-thawed mouse morulae. Experientia 37, 187–188 (1981). https://doi.org/10.1007/BF01963224
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DOI: https://doi.org/10.1007/BF01963224