Abstract
The presence ofToxoplasma gondii in amniotic fluid was demonstrated using tissue culture in four of nine cases of congenital toxoplasmosis, whereasToxoplasma gondii antigen was undetectable using a sensitive enzyme immunoassay technique. Parasites were identified in monolayers four days after inoculation using an indirect immunofluorescence assay. Since tissue culture may provide evidence of infection within a few days, this method is proposed for early prenatal diagnosis of congenital toxoplasmosis.
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References
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Derouin, F., Thulliez, P., Candolfi, E. et al. Early prenatal diagnosis of congenital toxoplamosis using amniotic fluid samples and tissue culture. Eur. J. Clin. Microbiol. Infect. Dis. 7, 423–425 (1988). https://doi.org/10.1007/BF01962355
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DOI: https://doi.org/10.1007/BF01962355