Skip to main content
Log in

Comparison of the lysis centrifugation and radiometric blood culture systems for recovery of yeast

  • Notes
  • Published:
European Journal of Clinical Microbiology and Infectious Diseases Aims and scope Submit manuscript

Abstract

The performance of the Isolator (lysis centrifugation) and Bactec (radiometric) detection systems for the recovery of fungi from blood was studied prospectively by comparison of 2,188 paired cultures obtained at two geographically separated teaching hospitals. Eighty-three yeast isolates were recovered from 78 (3.6%) cultures that were obtained from 43 patients. Seventy-three (88%) yeast strains were recovered using the Isolator system, and 60 (72%) were recovered in the Bactec system. The average time for recovery of yeast was 2.3 days for the Isolator system and 3.1 days for the Bactec system. Optimal recovery can be accomplished through the use of both systems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Bille, J., Edson, R. S., Roberts, G. D. Clinical evaluation of the lysis-centrifugation blood culture system for the detection of fungemia and comparison with a conventional biphasic broth blood culture system. Journal of Clinical Microbiology 1984, 19: 126–128.

    PubMed  Google Scholar 

  2. Bodey, G. P. Fungal infection and fever of unknown origin in neutropenic patients. American Journal of Medicine 1986, Supplement 5C: 112–119.

    Google Scholar 

  3. Horn, R., Wong, B., Kiehn, T. E., Armstrong, D. Fungemia in a cancer hospital: changing frequency, earlier onset, and results of therapy. Reviews of Infectious Diseases 1985, 7: 646–655.

    PubMed  Google Scholar 

  4. Dorn, G. L., Land, G. A., Wilson, G. E. Improved blood culture technique based on centrifugation: clinical evaluation. Journal of Clinical Microbiology 1979, 9: 391–396.

    PubMed  Google Scholar 

  5. Bille, J., Stockman, L., Roberts, G. D., Horstmeier, C. D., Ilstrup, D. M. Evaluation of a lysis-centrifugation system for recovery of yeasts and filamentous fungi from blood. Journal of Clinical Microbiology 1983, 18: 469–471.

    PubMed  Google Scholar 

  6. Prevost, E., Bannister, E. Detection of yeast septicemia by biphasic and radiometric methods. Journal of Clinical Microbiology 1981, 13: 655–660.

    PubMed  Google Scholar 

  7. Brannon, P., Kiehn, T. E. Large-scale clinical comparison of the lysis-centrifugation and radiometric detection systems for blood culture. Journal of Clinical Microbiology 1985, 22: 951–954.

    PubMed  Google Scholar 

  8. Kellogg, J. A., Manzella, J. P., McConville, J. H. Clinical laboratory comparison of the 10-ml Isolator blood culture system with Bactec radiometric blood culture media. Journal of Clinical Microbiology 1984, 20: 618–623.

    PubMed  Google Scholar 

  9. McLaughlin, J. C., Hamilton, P., Scholes, J. V., Bartlett, R. C. Clinical laboratory comparison of lysis-centrifugation and Bactec radiometric blood culture techniques. Journal of Clinical Microbiology 1983, 18: 1027–1031.

    PubMed  Google Scholar 

  10. Ilstrup, D. M. Statistical methods employed in the study of blood culture media. In: Washington, J. A. (ed.): The detection of septicemia. CRC Press, West Palm Beach, FL, 1978, p. 31.

    Google Scholar 

  11. Kiehn, T. E., Wong, B., Edwards, F. F., Armstrong, D. Comparative recovery of bacteria and yeasts from lysiscentrifugation and a conventional blood culture system. Journal of Clinical Microbiology 1983, 18: 300–304.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Body, B.A., Pfaller, M.A., Durrer, J. et al. Comparison of the lysis centrifugation and radiometric blood culture systems for recovery of yeast. Eur. J. Clin. Microbiol. Infect. Dis. 7, 417–420 (1988). https://doi.org/10.1007/BF01962353

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01962353

Keywords

Navigation