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Comparative evaluation of a new molecular method for typingStaphylococcus epidermidis

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Abstract

The discriminatory powers of several techniques for typingStaphylococcus epidermidis were evaluated in an epidemiological study of bacteria isolated from intensive care patients and from neonates. Genomic DNA fingerprinting usingBcl/I restriction endonuclease was an effective epidemiological marker. The distinct restriction fragment profiles produced with this enzyme were highlighted with specific probes in a Southern blot technique. ClonedEscherichia coli rRNA gene probes proved to have lower discriminatory power and be less suitable for intraspecies typing. However, a panel of random genomicStaphylococcus epidermidis DNA clones provided almost the same level of discrimination as the DNA fingerprinting technique and also provided a clearer profile. DNA and gene fingerprinting techniques were reproducible and highly discriminatory compared to typing based on antigen and plasmid profiles, antibiotic susceptibility patterns and biotypes.

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Authors and Affiliations

  1. Karolinska Institute, Center for BioTechnology, Novum, S-141 52, Huddinge, Sweden

    J. Wilton & J. I. Flock

  2. Microbial Pathogenicity Research Group, Clinical Research Centre, Watford Road, HA1 3UJ, Harrow, Middlesex, UK

    J. Wilton

  3. Stockholm County Council Central Microbiological Laboratory, Box 70740, 10726, Stockholm, Sweden

    K. Jung, I. Vedin & B. Aronsson

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  1. J. Wilton
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  2. K. Jung
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  4. B. Aronsson
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  5. J. I. Flock
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Wilton, J., Jung, K., Vedin, I. et al. Comparative evaluation of a new molecular method for typingStaphylococcus epidermidis . Eur. J. Clin. Microbiol. Infect. Dis. 11, 515–521 (1992). https://doi.org/10.1007/BF01960806

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