Summary
In cultures with efficient aeration a maximum cell concentration (MCC) of 6×105 cells/ml (defined medium) and 5.5×106 cells/ml (broth) can be reached. By culturing within Millicells with excess supply of medium and efficient removal of waste products a physical limit for MCC of about 13×106 cells/ml is reached.
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Hellung-Larsen, P. Parameters affecting the maximum cell concentration ofTetrahymena. Experientia 44, 58–60 (1988). https://doi.org/10.1007/BF01960245
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DOI: https://doi.org/10.1007/BF01960245