Summary
A 142 base-pair satellite DNA from the mealworm beetle,Tenebrio molitor, has been cloned and sequenced. The satellite DNA is revealed by making a restriction digest of genomic DNA with either EcoRI or Hinfl, and constitutes approximately 49% of the genomic DNA. The presence of huge amounts of satellite DNA correlates well with the prominent blocks of heterochromatin found in tenebrionid beetles. A similar restriction digest ofXanthogaleruca luteola genomic DNA does not release a prominent satellite component.
References
John, B., and Miklos, G. L. G., Int. Rev. Cytol.58 (1979) 1.
Beridze, T., Satellite DNA. Springer-Verlag, Berlin 1986.
Blin, N., and Stafford, D. W., Nucl. Acids Res.3 (1976) 2303.
Maniatis, T., Fritsch, E. F., and Sambrook, J. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor 1982.
Southern, E. M., J. molec. Biol.98 (1975) 503.
Sanger, F., Nicklen, S., and Coulson, A. R., Proc. natl Acad. Sci. USA74 (1977) 5463.
Weith, A., Chromosoma91 (1985) 287.
Petitpierre, E., and Juan, C., 9th Int. Chrom. Conf., Marseilles. Progr. Abstr. (1986)144.
Smith, S. G., J. Morph.91 (1952) 325.
Smith, S. G., Chromosoma4 (1952) 585.
Smith, S. G., and Virkki, N., in: Animal Cytogenetics, vol. 3, Coleoptera, Insecta. 5. Ed. B. John. G. Borntraeger, Berlin 1978.
Crowson, R. A., A. Rev. Ent.5 (1960) 111.
Crowson, R. A., The Biology of the Coleoptera. Academic Press, London 1981.