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Modulation of carcinoembryonic antigen release by HT-29 colon carcinoma line in the presence of different agents

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In this study we followed the effects of various differentiating agents on the expression of carcinoembryonic antigen (CEA) released into the medium by a colon carcinoma cell line HT-29. Butyric acid 1 mM markedly increased the level of CEA (12-fold in comparison to control levels). 12-O-tetradecanoyl-phorbol-13-acetate (TPA) 50 ng/ml and 5-azacytidine 4×10−6M increased the amount of CEA, 2- and 1.5-fold respectively. On the other hand retinoic acid 10−5M, N methyl-formamide 1% and N,N hexamethylene bisacetamide 2.5 mM decreased CEA 2-, 4- and 3-fold respectively. Our results emphasize that various differentiating agents affect CEA levels differently. Thus changes in CEA levels appear not to be reliable as a marker of a more differentiated phenotype.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel

    J. Friedman, M. Seger, H. Levinsky & D. Allalouf

  2. Virology Unit, Hasharon Hospital, Petah Tiqva, Israel

    M. Seger

  3. Connective Tissue Research Unit, Gattegno Research Institute, Tel Aviv, Israel

    J. Friedman, H. Levinsky & D. Allalouf

Authors
  1. J. Friedman
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  2. M. Seger
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  3. H. Levinsky
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  4. D. Allalouf
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Friedman, J., Seger, M., Levinsky, H. et al. Modulation of carcinoembryonic antigen release by HT-29 colon carcinoma line in the presence of different agents. Experientia 43, 1121–1122 (1987). https://doi.org/10.1007/BF01956058

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  • DOI: https://doi.org/10.1007/BF01956058

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