Summary
A series of 20(R)-n-alkylpregn-5-en-3β-ols with 0 to 9 (but not with 12) C-atoms in the alkyl group induced formation of oogonia inPhytophthora cactorum. However, maturation of the oogonia into germinating oospores occurred only when the alkyl group had 4–7 C-atoms.
References
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All of the sterols used in this study except cholesterol were prepared by J. M. Joseph, Ph.D. Dissertation, 1980, Drexel University. The chromatographic and mass spectral properties of the sterols reisolated fromP. cactorum were similar to those reported by Joseph. See also Nes, W. R., Joseph, J. M., Landrey, J. R., and Conner, R. L., J. biol. Chem.255 (1980) 11815. Procedures for isolation and quantitation are to be found in Nes, W. D., Sanders, G. A., and Heftmann, E., Lipids16 (1981) 744. The control cultures were found to contain small amounts of cholesterol by GLC and GLC-MS as was the Difco agar (derived from red algae which synthesize cholesterol). Thus, the occurrence of a few oogonia in the control is presumably due to the remnants of cholesterol left in the agar after its industrial processing.
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W.R.N. thanks the National Institutes of Health for support through grant No. AM-12172. We also appreciate the help of Mr A. Stafford and of Dr W. Haddon of the USDA, Berkeley, in obtaining the mass spectral and some of the chromatographic data. Reference to a company and/or product named by the Department is only for purposes of information and does not imply approval or recommendation of the product to the exclusion of others which may also be suitable.
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David Nes, W., Nes, W.R. Disassociation of oogonia formation from oospore production inPhytophthora cactorum by sterols with varying side chain lengths. Experientia 39, 276–278 (1983). https://doi.org/10.1007/BF01955301
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DOI: https://doi.org/10.1007/BF01955301