Abstract
The influence of childhood pertussis infection and of purified pertussis toxin on histamine relase from human basophil leucocytes was investigated. Three different stimuli, the peptide N-formyl-Met-Phe (NFMP), anti-IgE, and the calciuminnophore A23187 were used to challenge the cells. When NFMP was the stimulus, histamine release in the control group (age 0.5–17 years) increased in an age-dependent fashion, whereas anti-IgE and A23187 stimulated release did not vary with age. During the convulsive state of pertussis infection there was a significant reduction of histamine release in response to 10 μM NFMP (from 9.5±1.4 [n=21] to 6.7±1.5 [n=19],P<0.05) and in response to 800 and 80 U/ml anti-IgE (from 28.5±5 [n=19] to 16.3±5 [n=13],P<0.05, and from 6.9±1.7 [n=16] to 2±0.8 [n=13],P<0.01), whereas histamine release stimulated by A23187 was unchanged compared to release in control children. In vitro pretreatment of basophils from healthy children and adults with pertussis toxin also inhibited histamine release. When NFMP was the stimulus, release was completely blocked by pertussis toxin with an IC50 of about 11 ng/ml whereas anti-IgE stimulated release was only inhibited by 20%–30% and release induced by A23187 was reduced to 40%–50% by toxin treatment. In conclusion we have demonstrated a functional impairment of histamine release during the convulsive state of pertussis and that this inhibition is likely to be mediated by pertussis toxin.
Similar content being viewed by others
Abbreviations
- NFMP:
-
N-formyl-Met-Phe
Referencen
Birnbauer L, Abramowitz J, Brown AM (1990) Receptor-effector coupling by G proteins. Biochim Biophys Acta 1031: 163–224
Bland JM, Holland WW, Elliot A (1974) The development of respiratory symptoms in a kohort of Kent school children. Bull Physiopathol Respir 10:699–716
Britten N, Wadsworth J (1986) Long term respiratory sequelae of whooping cough in a nationally representative sample. BMJ 292:441–444
Casolaro V, Spadaro G, Marone G (1990) Human basophil releaseability. VI. Changes in basophil releaseability in patients with allergic rhinitis or bronchial asthma. Am Rev Respir Dis 142:1108–1111
Chang IK, Gottshall RY (1972) Sensitization of mice by inhalation of antigen after infection withBordetella pertussis. Infect Immun 6:92–94
Clausen CR, Munoz J, Bergman RK (1969) Reaginic-type of antibody in mice stimulated by extracts ofBordetella pertussis J Immunol 103:768–777
Conroy MC, Adkinson NF, Lichtenstein LM (1977) Measurement of IgE on human basophils: relation to serum IgE and anti-IgE induced histamine release. J Immunol 118:1317–1321
Griese M, Sideropoulou O, Reinhardt D (1989) Impaired formation of the second messenger cAMP in mononuclear blood cells of children with pertussis. Pediatr Res 25:209–213
Griese M, Kusenbach G, Reinhardt D (1990) Histamine release test in comparison to standard tests in diagnosis of childhood allergic asthma. Ann Allergy 65:46–51
Howenstine M, Eigen H, Tepper R (1991) Pulmonary function in infants after pertussis. J Pediatr 118:563–566
Johnston IDA, Lambert HP, Anderson HR, Patel S (1983) Respiratory morbidity and lung function after whooping cough. Lancet II:1104–1108
Johnston IDA, Bland M, Ingram D, Anderson HR, Warner JO, Lambert HP (1986) Effect of whooping cough in infancy on subsequent lung function and bronchial reactivity. Am Rev Respir Dis 134:270–275
Marone G, Poto S, DiMartino L, Condorelli M (1986) Human basophil releasability. J Allergy Clin Immunol 77:377–383
Mota I (1963) Biological characterization of “mast cell sensitizing” antibodies. Life Sci 7:465–474
Munoz JJ, Paecock MG (1990) Action of pertussigen (pertussis toxin) on serum IgE and on Fcε receptors on lymphocytes. Cell Immunol 127:327–336
Munoz JJ, Arai H, Bergman RK, Sadowski PL (1981) Biological activities of crystalline pertussigen fromBordetella pertussis. Infect Immun 33:820–826
Nakamura T, Ui M (1983) Suppression of passive cutaneous anaphylaxis by pertussin toxin, an islet-activating protein, as a result of inhibition of histamine release from mast cells. Biochem Pharmacol 32:3435–3441
Patriarca PA, Biellik RJ, Sanden G, Burstyn DG, Mitchell PD, Sivlerman PR, Davis JP, Manclark CR (1988) Sensitivity and specificity of clinical case definitions of pertussis. Am J Publ Health 78:833–836
Plan MS, Dobbs LG (1990) Pertussis toxin inhibits terbutaline and ionomycin-stimulated secretion and terbutaline stimulated cAMP accumulation in alveolar type II cells. Am Rev Respir Dis 141:A633
Swansea Research Unit Royal College General Practioners (1985) Respiratory sequelae of whooping cough. BMJ 290: 1937–1940
Teculescu DB, Bruant A, Aubry C, Pham QT, Kuntz C, Deschamps JP, Manciaux M (1991) Pertussis in french adolescents: risk factors and respiratory sequels. Respiration 58:15–20
Tung RS, Lichtenstein LM (1981) Cyclic AMP agonist inhibition increases at low levels histamine release from human basophils. J Pharmacol Exp Ther 218:642–648
Ui M (1984) Islet activating protein, pertussis toxin: a probe for functions of the inhibitory guanine nucleotide regulatory component of adenylate cyclase. Trends Pharmacol Sci July: 277–279
Warner JA, Yancey KB, Maclashan DW (1987) The effect of pertussis toxin on mediator release from human basophils. J Immunol 139:161–165
Author information
Authors and Affiliations
Additional information
This work was supported by a grant from the Deutsche Forschungsgemeinschaft (Re 509/4-1)
Rights and permissions
About this article
Cite this article
Griese, M., Merkel, G., Feldmann, R. et al. Histamine release from basophils in childhood: Age dependency and inhibition by pertussis infection and pertussis toxin. Eur J Pediatr 152, 585–591 (1993). https://doi.org/10.1007/BF01954086
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01954086